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Title: Procalcitonin NH2-terminal cleavage peptide has no mitogenic effect on normal human osteoblast-like cells

Abstract

The NH2-terminal cleavage peptide of procalcitonin (N-proCT) recently was reported to be a bone cell mitogen. The authors have investigated the effect of N-proCT on the proliferation of normal human cells that have the phenotype of mature osteoblasts (hOB cells). N-proCT treatment for 24, 48, or 96 h in concentrations from 1 nM to 1 microM did not significantly increase (3H)thymidine uptake (means ranged from -19% to 38% of control, no significant differences) in hOB cells (6-10 cell strains per experiment) plated at four different densities. However, the hOB cells responded significantly to treatment with transforming growth factor {beta} (3 ng/ml), bovine insulin (300 micrograms/ml), or 30% fetal calf serum, which were included in all experiments as positive controls. The (3H)thymidine uptake data were confirmed in a direct cell count experiment tested at 96 h. Thus they data do not support the hypothesis that N-proCT is a potent mitogen for normal human osteoblasts.

Authors:
; ; ; ; ;  [1]
  1. Endocrine Research Unit, Mayo Clinic, Rochester, MN (USA)
Publication Date:
OSTI Identifier:
5464031
Resource Type:
Journal Article
Journal Name:
Journal of Bone and Mineral Research; (United States)
Additional Journal Information:
Journal Volume: 6:5; Journal ID: ISSN 0884-0431
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BONE CELLS; CELL PROLIFERATION; PEPTIDES; BIOLOGICAL FUNCTIONS; CALCITONIN; MAN; MITOGENS; PHENOTYPE; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; AZINES; CONNECTIVE TISSUE CELLS; HETEROCYCLIC COMPOUNDS; HORMONES; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PEPTIDE HORMONES; POLYPEPTIDES; PRIMATES; PROTEINS; PYRIMIDINES; RIBOSIDES; SOMATIC CELLS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Hassager, C, Bonde, S K, Anderson, M A, Rink, H, Spelsberg, T C, and Riggs, B L. Procalcitonin NH2-terminal cleavage peptide has no mitogenic effect on normal human osteoblast-like cells. United States: N. p., 1991. Web. doi:10.1002/jbmr.5650060510.
Hassager, C, Bonde, S K, Anderson, M A, Rink, H, Spelsberg, T C, & Riggs, B L. Procalcitonin NH2-terminal cleavage peptide has no mitogenic effect on normal human osteoblast-like cells. United States. https://doi.org/10.1002/jbmr.5650060510
Hassager, C, Bonde, S K, Anderson, M A, Rink, H, Spelsberg, T C, and Riggs, B L. 1991. "Procalcitonin NH2-terminal cleavage peptide has no mitogenic effect on normal human osteoblast-like cells". United States. https://doi.org/10.1002/jbmr.5650060510.
@article{osti_5464031,
title = {Procalcitonin NH2-terminal cleavage peptide has no mitogenic effect on normal human osteoblast-like cells},
author = {Hassager, C and Bonde, S K and Anderson, M A and Rink, H and Spelsberg, T C and Riggs, B L},
abstractNote = {The NH2-terminal cleavage peptide of procalcitonin (N-proCT) recently was reported to be a bone cell mitogen. The authors have investigated the effect of N-proCT on the proliferation of normal human cells that have the phenotype of mature osteoblasts (hOB cells). N-proCT treatment for 24, 48, or 96 h in concentrations from 1 nM to 1 microM did not significantly increase (3H)thymidine uptake (means ranged from -19% to 38% of control, no significant differences) in hOB cells (6-10 cell strains per experiment) plated at four different densities. However, the hOB cells responded significantly to treatment with transforming growth factor {beta} (3 ng/ml), bovine insulin (300 micrograms/ml), or 30% fetal calf serum, which were included in all experiments as positive controls. The (3H)thymidine uptake data were confirmed in a direct cell count experiment tested at 96 h. Thus they data do not support the hypothesis that N-proCT is a potent mitogen for normal human osteoblasts.},
doi = {10.1002/jbmr.5650060510},
url = {https://www.osti.gov/biblio/5464031}, journal = {Journal of Bone and Mineral Research; (United States)},
issn = {0884-0431},
number = ,
volume = 6:5,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1991},
month = {Wed May 01 00:00:00 EDT 1991}
}