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Title: Detection of induced DNA strand breaks with improved sensitivity in human cells

When mixtures of two cell populations labeled with different radionuclides are tested with DNA unwinding technique, an accurate comparison with regard to DNA strand breaks can be made. In mixtures of irradiated and control cells, effects were detected by this technique down to a dose of 1 rad. This corresponds to 10 to 20 DNA single-strand breaks per cell, or less than one break per chromosome.
Authors:
Publication Date:
OSTI Identifier:
5463802
Resource Type:
Journal Article
Resource Relation:
Journal Name: Radiat. Res.; (United States); Journal Volume: 81:3
Research Org:
Univ. of Uppsala, Sweden
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; COBALT 60; GENETIC RADIATION EFFECTS; DNA; RADIOSENSITIVITY; GAMMA RADIATION; STRAND BREAKS; RADIOINDUCTION; CELL CULTURES; CHROMOSOMES; DOSE-RESPONSE RELATIONSHIPS; EXPERIMENTAL DATA; HELA CELLS; ISOLATED VALUES; ISOTOPE APPLICATIONS; POPULATIONS; THYMIDINE; TRITIUM; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; COBALT ISOTOPES; DATA; DATA FORMS; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LIGHT NUCLEI; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; NUMERICAL DATA; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RIBOSIDES; YEARS LIVING RADIOISOTOPES 560121* -- Radiation Effects on Cells-- External Source-- (-1987); 550400 -- Genetics; 550300 -- Cytology