Detection of induced DNA strand breaks with improved sensitivity in human cells

Journal Article · Sat Mar 01 00:00:00 EST 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575208· OSTI ID:5463802

Rydberg, B

When mixtures of two cell populations labeled with different radionuclides are tested with DNA unwinding technique, an accurate comparison with regard to DNA strand breaks can be made. In mixtures of irradiated and control cells, effects were detected by this technique down to a dose of 1 rad. This corresponds to 10 to 20 DNA single-strand breaks per cell, or less than one break per chromosome.

• Research Organization: Univ. of Uppsala, Sweden
• OSTI ID: 5463802
• Journal Information: Radiat. Res.; (United States), Vol. 81:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
COBALT 60
GENETIC RADIATION EFFECTS
DNA
RADIOSENSITIVITY
GAMMA RADIATION
STRAND BREAKS
RADIOINDUCTION
CELL CULTURES
CHROMOSOMES
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
HELA CELLS
ISOLATED VALUES
ISOTOPE APPLICATIONS
POPULATIONS
THYMIDINE
TRITIUM
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
COBALT ISOTOPES
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RIBOSIDES
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)
550400 - Genetics
550300 - Cytology