Use of stable isotopes of selenium in human metabolic studies: development of analytical methodology

Journal Article · Tue Dec 01 00:00:00 EST 1981 · Am. J. Clin. Nutr.; (United States)

OSTI ID:5462682

Janghorbani, M; Ting, B T; Young, V R

Use of stable isotopes of selenium in relation to enrichment of diets for studies of selenium absorption and metabolism in human subjects is discussed. A method based on radiochemical neutron activation analysis is described which allows accurate measurement of stable isotopes 74Se, 76Se, and 80Se in matrices (feces, plasma, red blood cells, and urine) of interest in metabolic studies. We show that these isotopes can routinely be measured with analytical precision and accuracy of 10% in samples of available size. This precision and accuracy is satisfactory for conduct of many nutritional experiments concerned with gastrointestinal absorption, plasma and red cell selenium turnover, and urinary excretion of the element, using an oral dose of 74Se. Original, experimental data are presented to illustrate the degree of enrichment of 74Se in feces, plasma, and urine after a single oral ingestion of 50 micrograms of the isotope with a breakfast meal in healthy young men.

• Research Organization: Department of Nutrition, Massachusetts Institute of Technology, Cambridge
• OSTI ID: 5462682
• Journal Information: Am. J. Clin. Nutr.; (United States), Vol. 34:12
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
SELENIUM ISOTOPES
INTESTINAL ABSORPTION
METABOLISM
BLOOD PLASMA
ERYTHROCYTES
FECES
MAN
ORAL ADMINISTRATION
SELENIUM 74
SELENIUM 76
SELENIUM 80
TRACER TECHNIQUES
URINE
ABSORPTION
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD CELLS
BODY FLUIDS
EVEN-EVEN NUCLEI
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
PRIMATES
RADIOISOTOPES
STABLE ISOTOPES
UPTAKE
VERTEBRATES
WASTES
560171* - Radiation Effects- Nuclide Kinetics & Toxicology- Man- (-1987)
550501 - Metabolism- Tracer Techniques