Thymic cell activity of thiouracil-treated chicks
Thymus glands of chickens fed a diet containing .1% thiouracil from day of hatch are less well developed than those of controls. Thymus weights are reduced, and the cortical and medullary areas are found to have poor cellularity upon histological examination. Fewer mitotic figures are observed in the thymic cortices of thiouracil-treated chickens, and indications of regression at an early age are noticeable in their medullae. The phytohemmagglutin-M response of peripheral blood lymphocytes (PBL) is reduced in thiouracil-fed chicks. However, PBL from thiouracil-treated chicks do not produce lower graft versus host responses (GvH). Thymic cells from 1 and 3 week-old chicks were cultured in vitro with BPMI 1640 medium for short lengths of time. The uptake of /sup 3/H-thymidine (/sup 3/HTdR) was measured after 1 and 4 hr incubation periods at 40/sup 0/C in a humidified 5% CO/sub 2/ atmosphere. There was more uptake of /sup 3/HTdR by thymic cells from control chicks after both 1 and 4 hr of incubation. Autoradiographic studies of thymic cells labeled in vitro with /sup 3/HTdR were also conducted. The T cells from chicks treated with thiouracil from day of hatch are less active than those from control chicks. But the functional ability of the T-cell subpopulation involved in GvH is not altered. A thiouracil-induced hypothyroid state would appear to affect thymic development in young chickens.
- Research Organization:
- Mississippi State Univ., State College
- OSTI ID:
- 5457970
- Report Number(s):
- CONF-8604222-; TRN: 86-028558
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
THIOURACIL
BIOLOGICAL EFFECTS
THYMUS
AUTORADIOGRAPHY
SENSITIVITY
BLOOD
CELL CULTURES
CHICKENS
DIET
GRAFT-HOST REACTION
LYMPHOCYTES
THYMIDINE
THYMUS CELLS
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
ANTITHYROID DRUGS
AZINES
BIOLOGICAL MATERIALS
BIRDS
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DRUGS
FOWL
HETEROCYCLIC COMPOUNDS
HORMONE ANTAGONISTS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHATIC SYSTEM
MATERIALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
THIOLS
URACILS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550201 - Biochemistry- Tracer Techniques