Characterization studies on cadmium-mycophosphatin from the mushroom Agaricus macrosporus
A low molecular weight Cd-binding phosphoglycoprotein, cadmium-mycophosphatin, has been isolated from the mushroom Agaricus macrosporus. This protein has a molecular weight of 12,000 dalton and contains no sulfur but a high amount of acid amino acids (Glu, Asp), and carbohydrates (glucose, galactose). Cadmium-mycophosphatin has an isoelectric point less than pH 2, binds cadmium with a dissociation constant of K/sub D/ = 1.59 x 10 M (pK/sub D/ = 6.8) and is saturated with 13.5 mole Cd/mole, all Cd-binding sites being equivalent. It is suggested that Cd is bound by phosphoserine groups, similar relations being known from calcium-binding proteins in animals. From A. macrosporus four other low-molecular weight glycoproteins have been isolated which contain sulfur and bind cadmium and copper. The biological significance of these Cd-binding proteins is discussed.
- Research Organization:
- Universitaet des Saarlandes, Saarbruecken, West Germany
- OSTI ID:
- 5454732
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 65
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
METALLOPROTEINS
BIOCHEMISTRY
CHEMICAL PROPERTIES
ABSORPTION SPECTROSCOPY
CADMIUM
MUSHROOMS
CHEMISTRY
ELEMENTS
FUNGI
METALS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
SPECTROSCOPY
550200* - Biochemistry
560302 - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)