Irreversible conversion of the physical state of herpes simplex virus preceding inactivation by thermal or antibody treatment
The buoyant density characteristics of infectious particles of herpes simplex virus types 1 and 2 were studied by centrifugation in sucrose and cesium chloride density gradients with a high resolution and satisfactory infectivity recovery. It was shown that two populations of infectious virions differing in buoyant density coexisted, the difference being slight but definite. The ratio of heavy (H) to light (L) viral particles varied depending upon the solute used, the strains of virus, and the cell origin. Circumstances favoring degradation of viral infectivity tended to increase the H portion. Incubation at 37 degrees C largely converted L to H, and heating at 45 degrees C converted all virions to H without infectivity. The L to H conversion was irreversible, and no populations intermediate between L and H were clearly observed. Inactivation by UV light irradiation did not affect the density pattern. That H was not an artefact due to penetration of solutes, osmotic pressure, viral aggregation, or loss of the envelope was shown experimentally. A difference in the outer shape of particles between negatively stained L and H populations was demonstrated by electron microscopy. Both cell-released and cell-bound herpes simplex virus particles gave essentially the same result with respect to the above characteristics. The effect of limiting dilutions of antiserum was similar to that of mild thermal treatment, in that denser virions increased parallel to a decrease in less dense virions. Sensitization with early immunoglobulin G, composed mainly of complement-requiring neutralizing antibody, caused the density transition, and subsequent addition of complement resulted in a further increase in the buoyant density of the sensitized virions.
- OSTI ID:
- 5451637
- Journal Information:
- J. Virol.; (United States), Vol. 38:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HERPES SIMPLEX
INFECTIVITY
ANTIBODIES
CENTRIFUGATION
COMPARATIVE EVALUATIONS
DENSITY
ELECTRON MICROSCOPY
IMMUNE SERUMS
MEASURING METHODS
PARTICLES
QUANTITATIVE CHEMICAL ANALYSIS
TEMPERATURE EFFECTS
ULTRAVIOLET RADIATION
CHEMICAL ANALYSIS
DISEASES
ELECTROMAGNETIC RADIATION
INFECTIOUS DISEASES
MICROSCOPY
PHYSICAL PROPERTIES
RADIATIONS
SEPARATION PROCESSES
SKIN DISEASES
VIRAL DISEASES
550200* - Biochemistry
550700 - Microbiology