A histochemical and X-ray microanalysis study of calcium changes in insect flight muscle degeneration in Solenopsis, the queen fire ant
Potassium pyroantimonate histochemistry, coupled with ethyleneglycoltetraacetic acid (EGTA)-chelation and X-ray microprobe analysis, was employed to localize intracellular calcium binding sites in the normal and degenerating flight musculature in queens of Solenopsis, the fire ant. In normal animals, calcium distribution was light to moderate within myofibrils and mitochondria. In the early contracture stages of the insemination-induced degeneration, both myofilament and mitochondrial calcium loading was markedly increased. In the terminal stages of myofibril breakdown, only Z-lines (isolated or in clusters) with an associated filamentous residue persisted. These complexes were also intensely calcium positive. This study further documents the presence of increased sarcoplasmic calcium during muscle necrosis. Surface membrane defects, mitochondrial calcium overload, and calcium-activated proteases may all be involved in this ''normal'' breakdown process.
- Research Organization:
- Department of Microscopic Anatomy, College of Dentistry, Dallas, Texas
- OSTI ID:
- 5449276
- Journal Information:
- J. Histochem. Cytochem.; (United States), Vol. 30:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CALCIUM
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
ELECTRON PROBES
INSECTS
INVERTEBRATES
MITOCHONDRIA
MUSCLES
PATHOLOGICAL CHANGES
X-RAY EMISSION ANALYSIS
ALKALINE EARTH METALS
ANIMALS
ARTHROPODS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
ELEMENTS
KINETICS
METALS
NONDESTRUCTIVE ANALYSIS
ORGANOIDS
PROBES
REACTION KINETICS
551001* - Physiological Systems- Tracer Techniques