Uptake of (N-Me-3H)-choline by synaptosomes from the central nervous system of Locusta migratoria
The accumulation of 3H-choline by isolated synaptosomes from the central nervous system of locust was studied at concentrations varying from 0.05 to 40 microM. Kinetic analysis of the saturable process revealed a high-affinity and a low-affinity system. The high-affinity uptake was competitively inhibited by hemicholinium-3 and was absolutely dependent on external sodium. Elevated potassium concentrations inhibited choline uptake. The choline uptake by insect synaptosomes was found to be remarkably resistant to a variety of metabolic inhibitors. The reduced choline uptake under depolarizing conditions (high potassium concentration or veratridine) in the absence of calcium implies that electrochemical gradients are important for high-affinity choline uptake. Depolarization of preloaded synaptosomes under appropriate conditions resulted in a significant release of newly accumulated choline radioactivity.
- Research Organization:
- University of Osnabrueck, Zoophysiologie/Zellphysiologie, Federal Republic of Germany
- OSTI ID:
- 5449265
- Journal Information:
- J. Neurobiol.; (United States), Vol. 13:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CENTRAL NERVOUS SYSTEM
PHYSIOLOGY
CHOLINE
UPTAKE
BIOCHEMICAL REACTION KINETICS
GRASSHOPPERS
MEMBRANE TRANSPORT
POTASSIUM
ALCOHOLS
ALKALI METALS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ARTHROPODS
DRUGS
ELEMENTS
HYDROXY COMPOUNDS
INSECTS
INVERTEBRATES
KINETICS
LIPOTROPIC FACTORS
METALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORTHOPTERA
QUATERNARY COMPOUNDS
REACTION KINETICS
551001* - Physiological Systems- Tracer Techniques
550501 - Metabolism- Tracer Techniques