Sister chromatid exchange frequency in human epidermal cells in culture treated with 8-methoxypsoralen and long-wave UV radiation
The effects of 8-methoxypsoralen with long-wave ultraviolet radiation on the sister chromatid exchange frequency in human epidermal cells in culture was investigated. With a constant amount of radiation the number of exchanges increased in an approximately linear manner with increasing concentrations of 8-methoxypsoralen up to 0.3 micrograms/ml. Above this concentration there were fewer dividing cells and an apparent departure from linearity in the dose-response curve. These results show that 8-methoxypsoralen concentrations equivalent to those found in the serum of patients undergoing photochemotherapy, in conjunction with UVA radiation, cause striking increases in sister chromatid exchange frequency in human epidermal cells in vitro.
- Research Organization:
- Department of Pathology, Ninewells Hospital and Medical School, Dundee, Scotland
- OSTI ID:
- 5448855
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 78:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of nickel sulfate, lead sulfate, and sodium arsenite alone and with UV light on sister chromatid exchanges in cultured human lymphocytes
Correlation of morphological transformation to sister chromatid exchanges induced by split doses of chemical or physical carcinogens on cultured Syrian hamster cells
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
EPIDERMIS
BIOLOGICAL RADIATION EFFECTS
PSORALEN
RADIOSENSITIVITY EFFECTS
CELL CULTURES
CHROMOSOMES
DOSE-RESPONSE RELATIONSHIPS
FAR ULTRAVIOLET RADIATION
IN VITRO
MUTATION FREQUENCY
RADIOINDUCTION
SISTER CHROMATID EXCHANGES
ANIMAL TISSUES
ANTICOAGULANTS
BIOLOGICAL EFFECTS
BODY
CHROMOSOMAL ABERRATIONS
COUMARINS
DRUGS
ELECTROMAGNETIC RADIATION
EPITHELIUM
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
SKIN
TISSUES
ULTRAVIOLET RADIATION
560121* - Radiation Effects on Cells- External Source- (-1987)
560111 - Radiation Effects on Biochemicals- In Vitro- (-1987)
550200 - Biochemistry