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Title: 12-O-tetradecanoylphorbol-13-acetate disrupts actin filaments and focal contacts and enhances binding of fibronectin-coated latex beads to 3T3-L1 cells

Abstract

The effect of a tumor-promoting phorbol ester on the binding of fibronectin-coated beads to 3T3-L1 cells was studied to clarify the relationship between the binding of fibronectin to the cells, cell adhesion, and the organization of actin filaments. Interference-reflection microscopy revealed focal contacts of 3T3-L1 cells with the substratum. Stress fibers observed after rhodomine-phalloidin staining were well-developed in the cells. Treatment of the cells for 20 min with 12-O-tetradecanoylphorbol-13-acetate (TPA), but not with phorbol, disrupted focal contacts and caused a reorganization of stress fibers to generate actin ribbons. Treatment of the cells with TPA enhanced the binding of beads coated with human plasma fibronectin to the cells, as observed after incubation for 6 h with the beads. The TPA-induced increase in the percentage of cells with bound beads was dependent on the duration of treatment with TPA and on the concentration of TPA. Treatment of the cells with TPA also enhanced proliferation of cells in a dose-dependent manner. Furthermore, treatment of the cells with phorbol did not enhance the binding of beads coated with fibronectin. These results suggest that TPA specifically enhances the binding of fibronectin-coated beads to 3T3-L1 cells, and that TPA-induced binding of the beads may be relatedmore » to disruption of focal contacts and reorganization of actin filaments.« less

Authors:
; ;  [1]
  1. Hiroshima Univ. School of Dentistry (Japan)
Publication Date:
OSTI Identifier:
5447560
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research; (United States)
Additional Journal Information:
Journal Volume: 178:2; Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; FIBROBLASTS; ADHESION; PHORBOL ESTERS; BIOLOGICAL EFFECTS; SCLEROPROTEINS; BIOCHEMISTRY; ACTIN; COAGULANTS; DOSE-RESPONSE RELATIONSHIPS; RECEPTORS; SCANNING ELECTRON MICROSCOPY; ANIMAL CELLS; CARCINOGENS; CHEMISTRY; CONNECTIVE TISSUE CELLS; DRUGS; ELECTRON MICROSCOPY; ESTERS; HEMATOLOGIC AGENTS; MEMBRANE PROTEINS; MICROSCOPY; ORGANIC COMPOUNDS; PROTEINS; SOMATIC CELLS; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Shiba, Yoshiki, Sasaki, Yasuto, and Kanno, Yoshinobu. 12-O-tetradecanoylphorbol-13-acetate disrupts actin filaments and focal contacts and enhances binding of fibronectin-coated latex beads to 3T3-L1 cells. United States: N. p., 1988. Web. doi:10.1016/0014-4827(88)90394-1.
Shiba, Yoshiki, Sasaki, Yasuto, & Kanno, Yoshinobu. 12-O-tetradecanoylphorbol-13-acetate disrupts actin filaments and focal contacts and enhances binding of fibronectin-coated latex beads to 3T3-L1 cells. United States. https://doi.org/10.1016/0014-4827(88)90394-1
Shiba, Yoshiki, Sasaki, Yasuto, and Kanno, Yoshinobu. 1988. "12-O-tetradecanoylphorbol-13-acetate disrupts actin filaments and focal contacts and enhances binding of fibronectin-coated latex beads to 3T3-L1 cells". United States. https://doi.org/10.1016/0014-4827(88)90394-1.
@article{osti_5447560,
title = {12-O-tetradecanoylphorbol-13-acetate disrupts actin filaments and focal contacts and enhances binding of fibronectin-coated latex beads to 3T3-L1 cells},
author = {Shiba, Yoshiki and Sasaki, Yasuto and Kanno, Yoshinobu},
abstractNote = {The effect of a tumor-promoting phorbol ester on the binding of fibronectin-coated beads to 3T3-L1 cells was studied to clarify the relationship between the binding of fibronectin to the cells, cell adhesion, and the organization of actin filaments. Interference-reflection microscopy revealed focal contacts of 3T3-L1 cells with the substratum. Stress fibers observed after rhodomine-phalloidin staining were well-developed in the cells. Treatment of the cells for 20 min with 12-O-tetradecanoylphorbol-13-acetate (TPA), but not with phorbol, disrupted focal contacts and caused a reorganization of stress fibers to generate actin ribbons. Treatment of the cells with TPA enhanced the binding of beads coated with human plasma fibronectin to the cells, as observed after incubation for 6 h with the beads. The TPA-induced increase in the percentage of cells with bound beads was dependent on the duration of treatment with TPA and on the concentration of TPA. Treatment of the cells with TPA also enhanced proliferation of cells in a dose-dependent manner. Furthermore, treatment of the cells with phorbol did not enhance the binding of beads coated with fibronectin. These results suggest that TPA specifically enhances the binding of fibronectin-coated beads to 3T3-L1 cells, and that TPA-induced binding of the beads may be related to disruption of focal contacts and reorganization of actin filaments.},
doi = {10.1016/0014-4827(88)90394-1},
url = {https://www.osti.gov/biblio/5447560}, journal = {Experimental Cell Research; (United States)},
issn = {0014-4827},
number = ,
volume = 178:2,
place = {United States},
year = {Sat Oct 01 00:00:00 EDT 1988},
month = {Sat Oct 01 00:00:00 EDT 1988}
}