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Title: Small-angle neutron scattering studies from solutions of bovine nasal cartilage proteoglycan

Small-angle neutron scattering, SANS, of the proteoglycan subunit of bovine nasal cartilage in 0.15N LiCl at 25/sup 0/C yielded the radius of gyration, R/sub g/, radius of gyration of the cross-section, R/sub q/, persistence length, a, and the molecular weight, M. The following values were obtained: M = 3.9 x 10/sup 6/, R/sub g/ = 745 A, R/sub q/ = 34.6 A and a = 35.2 A. These values compare favorably with those that were obtained from small angle x-ray scattering, SAXS, of a similar extract. The scattering curve of the proteoglycan subunit in D/sub 2/O showed a characteristic broad peak in the specified angular range similar to that observed from SAXS, thus confirming the polyelectrolyte nature of the proteoglycan. 15 refs., 3 figs., 1 tab. (DT)
Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
5446726
Report Number(s):
CONF-850871-7
ON: DE85016935
DOE Contract Number:
AC05-84OR21400
Resource Type:
Conference
Resource Relation:
Conference: International conference on neutron scattering, Santa Fe, NM, USA, 19 Aug 1985; Other Information: Portions of this document are illegible in microfiche products
Research Org:
Stevens Inst. of Tech., Hoboken, NJ (USA). Dept. of Chemistry and Chemical Engineering; Rockefeller Univ., New York (USA); Oak Ridge National Lab., TN (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; MUCOPOLYSACCHARIDES; NEUTRON DIFFRACTION; AQUEOUS SOLUTIONS; CARTILAGE; COWS; NOSE; PROTEIN STRUCTURE; AMINES; ANIMAL TISSUES; ANIMALS; BODY; BODY AREAS; CARBOHYDRATES; CATTLE; COHERENT SCATTERING; CONNECTIVE TISSUE; DIFFRACTION; DISPERSIONS; DOMESTIC ANIMALS; FACE; HEAD; MAMMALS; MIXTURES; ORGANIC COMPOUNDS; POLYSACCHARIDES; RESPIRATORY SYSTEM; RUMINANTS; SACCHARIDES; SCATTERING; SOLUTIONS; TISSUES; VERTEBRATES 550200* -- Biochemistry; 400101 -- Activation, Nuclear Reaction, Radiometric & Radiochemical Procedures