Presence and in vivo biosynthesis of fragments of CPP (the C-terminal glycopeptide of the rat vasopressin precursor) in the hypothalamo-neurohypophyseal system

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Peptides (Fayetteville, N.Y.); (United States)

DOI:https://doi.org/10.1016/0196-9781(87)90054-4· OSTI ID:5445680

Seger, M A; Burbach, J P

The existence and rate of formation of fragments of the 39-residue C-terminal glycopeptide of propressophysin (CPP1-39) was investigated in the hypothalamo-neurohypophyseal system. Newly-prepared antisera to CPP were used to confirm the existence of smaller C-terminal fragments derived from CPP1-39. Radiolabelled fucose was injected into rats in vivo into the area of the supraoptic nucleus, and the labelled peptides formed in the neurohypophysis were examined at various time intervals up to five weeks after the injection. The products derived from the neurohypophyseal hormone precursors were separated by high-performance liquid chromatography. The level of the major immunoreactive C-terminal fragment (CPP22-39) was constant and represented about 5% of the intact CPP1-39 in the neurohypophysis. The appearance of newly-synthesized N-terminal fragment of CPP1-39 occurred only after 3 or 4 days. This fucose labelled fragment increased slowly thereafter until it reached the same level as the CPP C-terminal fragment immunoreactivity between 21 and 28 days after injection. The results suggest that CPP1-39 is extremely stable in the hypothalamo-neurohypophyseal neurons, and that the cleavage at Arg21-Leu22 is a delayed proteolytic event in the magnocellular neurons of the SON.

• Research Organization: Medical Faculty Univ. of Utrecht (The Netherlands)
• OSTI ID: 5445680
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Vol. 8:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
BIOSYNTHESIS
ANTIBODIES
CARBOXYPEPTIDASES
HEXOSES
IMMUNE REACTIONS
LIQUID COLUMN CHROMATOGRAPHY
NERVE CELLS
PRECURSOR
RADIOIMMUNOASSAY
RATS
TRITIUM COMPOUNDS
TYROSINE
VASOPRESSIN
AMINO ACIDS
ANIMAL CELLS
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ENZYMES
HORMONES
HYDROLASES
HYDROXY ACIDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PITUITARY HORMONES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques