Molecular cloning, chromosomal mapping, and functional expression of human brain glutamate receptors
- Univ. of California, San Diego, La Jolla (United States)
- Univ. of California, Irvine (United States)
- Salk Inst. for Biological Studies, La Jolla, CA (United States)
A full-length cDNA clone encoding a glutamate receptor was isolated from a human brain cDNA library, and the gene product was characterized after expression in Xenopus oocytes. Degenerate PCR primers to conserved regions of published rat brain glutamate receptor sequences amplified a 1-kilobase fragment from a human brain cDNA library. This fragment was used as a probe for subsequent hybridization screening. Two clones were isolated that, based on sequence information, code for different receptors: a 3-kilobase clone, HBGR1, contains a full-length glutamate receptor cDNA highly homologous to the rat brain clone GluR1, and a second clone, HBGR2, contains approximately two-thirds of the coding region of a receptor homologous to rat brain clone GluR2. Southern and PCr analysis of a somatic cell-hybrid panel mapped HBGR1 to human chromosome 5q31.3-33.3 and mapped HBGR2 to chromosome 4q25-34.3. Xenopus oocytes injected with in vitro-synthesized HBGR1 cRNA expressed currents activated by glutamate receptor agonists. These results indicate that clone HBGR1 codes for a glutamate receptor of the kainate subtype cognate to members of the glutamate receptor family from rodent brain.
- OSTI ID:
- 5445315
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 89:4; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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550400* - Genetics