Method for studying the mutagenicity of some gaseous compounds in Salmonella typhimurium

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Environ. Mol. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2850110108· OSTI ID:5439902

Victorin, K; Stahlberg, M

A dynamic flow-through exposure system was designed for mutagenicity studies of gaseous compounds in Salmonella. Salmonella typhimurium strain TA100 was the primary tester strain. The dose ranges were 0.5-20% of vinyl chloride, ethene, propene, and 1,3-butadiene, 1-200 ppm of ethylene oxide, 0.5-20 ppm of nitrogen dioxide, and 0.1-3.5 ppm of ozone. The gas flow rate was 250, 500, or 1000 ml/min, and the exposure time was 6 or 7 hours. Of the tested gases, vinyl chloride, ethylene oxide, and nitrogen dioxide were mutagenic. Ethene, propene, and 1,3-butadiene were not mutagenic in this system. Ozone is bacteriotoxic, and no mutagenic effect could be demonstrated in the nontoxic dose range. The exposure system was considered suitable for studies on gaseous chemicals.

• Research Organization: National Institute of Environmental Medicine, Stockholm (Sweden)
• OSTI ID: 5439902
• Journal Information: Environ. Mol. Mutagen.; (United States), Vol. 11:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BUTADIENE
MUTAGEN SCREENING
ETHYLENE
NITROGEN DIOXIDE
OZONE
PROPYLENE
VINYL CHLORIDE
DOSE-RESPONSE RELATIONSHIPS
GASES
SALMONELLA TYPHIMURIUM
ALKENES
BACTERIA
CHALCOGENIDES
CHLORINATED ALIPHATIC HYDROCARBONS
DIENES
FLUIDS
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
POLYENES
SALMONELLA
SCREENING
560300* - Chemicals Metabolism & Toxicology