Consensus topography in the ATP binding site of the simian virus 40 and polyomavirus large tumor antigens
The location and sequence composition of a consensus element of the nucleotide binding site in both simian virus 40 (SV40) and polyomavirus (PyV) large tumor antigens (T antigens) can be predicted with the assistance of a computer-based pattern-matching system, ARIADNE. The latter was used to optimally align elements of T antigen primary sequence and predicted secondary structure with a descriptor for a mononucleotide binding fold. Additional consensus elements of the nucleotide binding site in these two proteins were derived from comparisons of T antigen primary and predicted secondary structures with x-ray structures of the nucleotide binding sites in four otherwise unrelated proteins. Each of these elements was predicted to be encompassed within a 110-residue segment that is highly conserved between the two T antigens residues 418-528 in SV 40 T antigen and residues 565-675 in PyV. Results of biochemical and immunologic experiments on the nucleotide binding behavior of these proteins using (/sup 32/P)-Amp-labeled SV40 T antigen, were found to be consistent with these predictions. Taken together, the latter have resulted in a topological model of the ATP binding site in these two oncogene products.
- Research Organization:
- Harvard Medical School, Boston, MA (United States)
- OSTI ID:
- 5437394
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGENS
CROSS-LINKING
STRUCTURAL CHEMICAL ANALYSIS
X-RAY DIFFRACTION
NUCLEOTIDES
PHOSPHORUS 32
TRACER TECHNIQUES
AMP
ATP
COMPUTER GRAPHICS
MONOCLONAL ANTIBODIES
NEOPLASMS
POLYOMA VIRUS
SIMIAN VIRUS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
COHERENT SCATTERING
DAYS LIVING RADIOISOTOPES
DIFFRACTION
DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
POLYMERIZATION
RADIOISOTOPES
SCATTERING
VIRUSES
550201* - Biochemistry- Tracer Techniques
550602 - Medicine- External Radiation in Diagnostics- (1980-)