T3 glycoprotein is functional although structurally distinct on human T-cell receptor. gamma. T lymphocytes

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.11.3817· OSTI ID:5436967

Krangel, M S; Bierer, B E; Devlin, P; Clabby, M; Strominger, J L; McLean, J; Brenner, M B

The T-cell receptor (TCR) ..gamma.. gene product occurs in association with T3 (CD3) polypeptides on the surface of human T lymphocytes. TCR ..gamma.. lymphocytes express arrays of T3 polypeptides distinct from those typically observed on TCR ..cap alpha beta.. lymphocytes. This report demonstrates that identical T3 ..gamma.., delta, and element of polypeptides are synthesized by TCR ..gamma.. lymphocytes and TCR ..cap alpha beta.. lymphocytes. However, the processing of T3 delta oligosaccharides is distinct in the two cell types. This observation may suggest distinct quaternary structures of these receptor complexes. Despite these structural differences, the T3 molecule on TCR ..gamma.. lymphocytes is functional. It is associated with and comodulates with TCR ..gamma.. and it serves as a substrate from protein kinase C-mediated phosphorylation. Anti-T3 monoclonal antibodies induce a rapid increase in cytoplasmic free calcium, indicating that the receptor complex is involved in signal transduction and triggering of TCR ..gamma.. lymphocytes.

• Research Organization: Harvard Medical School, Boston, MA (United States)
• OSTI ID: 5436967
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:12
• Country of Publication: United States
• Language: English

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