T helper cell cytotoxicity

Penna, A; Glasebrook, A

Title: T helper cell cytotoxicity

Full Record
Other Related Research

Abstract

It has recently been shown that helper T cells (Lyt2/sup -/, L3T4/sup +/) can express cytolytic activity when activated by antigen (Ag). The authors have studied the phenomenon of T helper cell cytotoxicity using cloned lines of Ag-reactive T cells and T hybrids. Cytotoxicity was determined by coculture of T cells with /sup 51/Cr-labelled Ag presenting cells (APC) and/or non-APC (bystander cells). A high frequency of Ag-specific L3T4/sup +/ T cell clones (> 90%) and hybrids (> 50%) were found to be cytotoxic. Cytotoxicity as determined by /sup 51/Cr release was maximal at 20 hr with little or no cytotoxicity detectable at 6 hr. Target cells, either APC or bystander cells, were killed provided the T cells were stimulated by Ag. Not all of the B cells used as APC were susceptible targets even if able to promote bystander killing. Monoclonal antibodies directed against L3T4, LFA-1 and T cell receptor molecules were able to block the cytotoxicity indicating a requirement for specific activation of the T cells. Cyclosporin A (CsA) reduced the cytotoxic activity of helper T hybrids and clones, while it did not affect the cytotoxic activity of Lyt2/sup +/, L3T4/sup -/ cytolytic T cell (CTL) clones. The delayedmore »« less

Authors:: Penna, A; Glasebrook, A

Publication Date:: Sat Mar 01 00:00:00 EST 1986

Research Org.:: Lilly Research Labs., La Jolla, CA

OSTI Identifier:: 5433573

Report Number(s):: CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 86-026755

Resource Type:: Conference

Journal Name:: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

Additional Journal Information:: Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; LYMPHOCYTES; BIOLOGICAL FUNCTIONS; RECEPTORS; ANTIGENS; CELL KILLING; CHROMIUM 51; CLONE CELLS; HYBRIDIZATION; IMMUNE REACTIONS; LYSIS; MONOCLONAL ANTIBODIES; TRACER TECHNIQUES; ANIMAL CELLS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CULTURES; CHROMIUM ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; FUNCTIONS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MATERIALS; MEMBRANE PROTEINS; NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; SOMATIC CELLS; 551001* - Physiological Systems- Tracer Techniques

Citation Formats


                    Penna, A, and Glasebrook, A. T helper cell cytotoxicity.  United States: N. p., 1986. 
        Web.

Copy to clipboard


                    Penna, A, & Glasebrook, A. T helper cell cytotoxicity.  United States.

Copy to clipboard


                    Penna, A, and Glasebrook, A. 1986.  
        "T helper cell cytotoxicity".  United States.

Copy to clipboard


                    
@article{osti_5433573,

  title        = {T helper cell cytotoxicity},

  author       = {Penna, A and Glasebrook, A},

  abstractNote = {It has recently been shown that helper T cells (Lyt2/sup -/, L3T4/sup +/) can express cytolytic activity when activated by antigen (Ag). The authors have studied the phenomenon of T helper cell cytotoxicity using cloned lines of Ag-reactive T cells and T hybrids. Cytotoxicity was determined by coculture of T cells with /sup 51/Cr-labelled Ag presenting cells (APC) and/or non-APC (bystander cells). A high frequency of Ag-specific L3T4/sup +/ T cell clones (> 90%) and hybrids (> 50%) were found to be cytotoxic. Cytotoxicity as determined by /sup 51/Cr release was maximal at 20 hr with little or no cytotoxicity detectable at 6 hr. Target cells, either APC or bystander cells, were killed provided the T cells were stimulated by Ag. Not all of the B cells used as APC were susceptible targets even if able to promote bystander killing. Monoclonal antibodies directed against L3T4, LFA-1 and T cell receptor molecules were able to block the cytotoxicity indicating a requirement for specific activation of the T cells. Cyclosporin A (CsA) reduced the cytotoxic activity of helper T hybrids and clones, while it did not affect the cytotoxic activity of Lyt2/sup +/, L3T4/sup -/ cytolytic T cell (CTL) clones. The delayed expression of cytotoxic activity, the lysis of bystander cells and inhibition by CsA suggest that the cytolytic mechanism is mediated by a soluble factor and different from the cytolytic mechanism of CTL. The phenomenon of cytotoxic T helper cells may be relevant to suppression of B cell immune responses in vivo.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5433573},
  journal      = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number       = ,

  volume       = 45:3,

  place        = {United States},

  year         = {Sat Mar 01 00:00:00 EST 1986},

  month        = {Sat Mar 01 00:00:00 EST 1986}

}

Copy to clipboard

Conference:

Other availability

Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Immunobiology of T cell responses to Mls-locus-disparate stimulator cells. III. Helper and cytolytic functions of cloned, Mls-reactive T cell lines

Journal Article Katz, M; Tite, J; Janeway, Jr, C - J. Immunol.; (United States)

Mls-specific T cell clones derived by limiting dilution were tested for cytotoxic activity in a lectin-dependent /sup 51/Cr-release assay. All the T cell clones tested were cytotoxic in such an assay in apparent contrast to previous reports (1, 2). However, only those target cells sensitive to cytolysis by other L3T4a/sup +/ cytolytic T cells (3) were killed by Mls-specific T cell clones in short term /sup 51/Cr-release assays, possibly explaining this discrepancy. All the T cell clones tested were L3T4a/sup +/,Lyt-2/sup -/ and stimulated B cells from Mls strains of mice to proliferate and secrete immunoglobulin. Furthermore, lysis of innocentmore »« less
Resistance of cytotoxic T lymphocytes to lysis by a clone of cytotoxic T lymphocytes

Journal Article Kranz, D; Eisen, H - Proc. Natl. Acad. Sci. U.S.A.; (United States)

To investigate how cytotoxic T lymphocytes (CTL) avoid killing themselves when they destroy target cells, the authors compared 20 different cell lines as target cells, including several CTL cell lines, for their susceptibility to lysis by CTL, measured by a standard /sup 51/Cr-release assay. Variations in recognition of this diverse set of target cells was circumvented by attaching to all of them a monoclonal antibody to the antigen-specific receptor of a cloned CTL cell line (clone 2C) and using the 2C cell line as the standard aggressor or effector cell. All of the nine tumor cell lines and the fourmore »« less
https://doi.org/10.1073/pnas.84.10.3375
DNA fragmentation: manifestation of target cell destruction mediated by cytotoxic T-cell lines, lymphotoxin-secreting helper T-cell clones, and cell-free lymphotoxin-containing supernatant

Journal Article Schmid, D; Tite, J; Ruddle, N - Proc. Natl. Acad. Sci. U.S.A.; (United States)

A Lyt-2/sup +/, trinitrophenyl-specific, lymphotoxin-secreting, cytotoxic T-cell line, PCl 55, mediates the digestion of target cell DNA into discretely sized fragments. This phenomenon manifests itself within 30 min after effector cell encounter as measured by the release of /sup 3/H counts from target cells prelabeled with (/sup 3/H)deoxythymidine and occurs even at very low effector to target cell ratios (0.25:1). A Lyt-1/sup +/, ovalbumin-specific, lymphotoxin-secreting T-helper cell clone, 5.9.24, is also able to mediate fragmentation of target cell DNA over a time course essentially indistinguishable from the cytotoxic T lymphocyte-mediated hit. Cell-free lymphotoxin-containing supernatants also cause release of DNA frommore »« less
https://doi.org/10.1073/pnas.83.6.1881
Features of target cell lysis by class I and class II MHC restricted cytolytic T lymphocytes

Journal Article Maimone, M; Morrison, L; Braciale, V; ... - J. Immunol.; (United States)

The lytic activity of influenza virus-specific muvine cytolytic T lymphocyte (CTL) clones that are restricted by either H-2K/D (class I) or H-2I (class II) major histocompatibility (MHC) locus products was compared on an influenza virus-infected target cell expressing both K/D and I locus products. With the use of two in vitro measurements of cytotoxicity, conventional /sup 51/Cr release, and detergent-releasable radiolabeled DNA (as a measure of nuclear disintegration in the early post-lethal hit period), the authors found no difference between class I and class II MHC-restricted CTL in the kinetics of target cell destruction. In addition, class II MHC-restricted antiviralmore »« less
Nature of the suppressor cells mediating prolonged graft survival after administration of extracted histocompatibility antigen and cyclosporine

Journal Article Yoshimura, N; Kahan, B - Transplantation; (United States)

Antigen-specific suppressor T cells are induced by donor histocompatibility antigen extracted from spleen cells with 3M KCl combined with cyclosporine (Ag-CsA). A single i.v. injection of 5 mg 3M-KCl-extracted donor Buffalo (Buf, RT1b) antigen (Ag) combined with a three day course of CsA prolonged renal allograft survival in Wistar-Furth (WFu, RT1u) hosts to a greater extent (MST 26.5 days) than CsA alone (MST 11.8 days). Peripheral blood lymphocytes (PBL) or spleen cells harvested from Ag-CsA-treated recipients ten days after transplantation inhibited the mixed lymphocyte reaction (MLR) between normal responder WFu cells and irradiated Buf cells (55.6% and 64.4% suppression, respectively,more »« less
https://doi.org/10.1097/00007890-198502000-00011

Similar Records