Characterization and isolation of a light driven sodium pump from membranes of Halobacterium halobium. Final technical progress report
We investigated three aspects of the light driven sodium pump (halorhodopsin, which appear to be crucial to our understanding of the mechanisms employed by Halobacterium halobium and to further investigate this unique system of energy conservation. We characterized the molecular mechanisms of transmembrane sodium transport in vesicles from H. halobium with particular reference to the mechanism of couplins of light energy to net sodium translocation. We develop procedures and techniques for extracting the components of the light driven sodium pump from membranes and incorporating them into artificial membrane systems. We examine the mechanism of conversion of bacteriorhodopsin from an active to an inactive form in membrane vesicles and to relate this alternative state of this pigment to the presence of the light driven sodium pump.
- Research Organization:
- Cornell Univ., Ithaca, NY (USA)
- DOE Contract Number:
- FG02-79ET00086
- OSTI ID:
- 5433421
- Report Number(s):
- DOE/ET/00086-1; ON: DE84005272
- Resource Relation:
- Other Information: Portions are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
14 SOLAR ENERGY
ASPARTIC ACID
MEMBRANE TRANSPORT
GLUTAMIC ACID
LIPOSOMES
BIOCHEMICAL REACTION KINETICS
PHOTOCHEMICAL REACTIONS
RHODOPSIN
BIOLOGICAL FUNCTIONS
SERINE
SODIUM
ABSORPTION SPECTROSCOPY
ACTIVATION ANALYSIS
ATP-ASE
BACTERIA
CYTOCHROMES
ELECTRON SPECTROSCOPY
NEUTRON REACTIONS
PHOSPHOTRANSFERASES
RAMAN SPECTRA
SODIUM 22
TRACER TECHNIQUES
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ALKALI METALS
AMINO ACIDS
BARYON REACTIONS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
ELEMENTS
ENZYMES
FUNCTIONS
HADRON REACTIONS
HYDROLASES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
METALS
MICROORGANISMS
NUCLEAR REACTIONS
NUCLEI
NUCLEON REACTIONS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHOHYDROLASES
PHOSPHORUS-GROUP TRANSFERASES
PIGMENTS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SODIUM ISOTOPES
SPECTRA
SPECTROSCOPY
TRANSFERASES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques
140505 - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)