Insulin reverses the growth retardation effect of phorbol ester in chicken embryos during organogenesis

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(89)90410-4· OSTI ID:5429408

Girbau, M; Bassas, L; Roth, J; de Pablo, F ^[1]

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1. Hospital Sta. Cruz y S. Pablo, Barcelona (Spain)

The tumor promoting phorbol esters can affect early embryonic development by causing interference with the normal pathways of cellular growth and differentiation. The present study was designed to: (a) define a time in organogenesis when a vertebrate embryo model, the chicken, was sensitive to the phorbol ester 12-0-tetradecanoil-13-acetate (TPA), and (b) attempt a rescue of the embryos disturbed by TPA with simultaneous addition of insulin. In embryos treated at days 2 and 3 of development, TPA caused dose-dependent mortality. Survivors were biochemically retarded as indicated by their decreased weight, protein, DNA, RNA, total creatine kinase, triglycerides, phospholipids and cholesterol contents. When intermediated doses of TPA were applied together with insulin the embryonic growth disturbance was largely antagonized. These data, generated with an in vivo whole embryo, support the strong link between the mode of action of insulin and signal transduction mechanisms typical of phorbol esters.

• OSTI ID: 5429408
• Journal Information: Life Sciences; (USA), Vol. 44:25; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL GROWTH
INHIBITION
CHICKENS
ONTOGENESIS
INSULIN
BIOLOGICAL EFFECTS
PHORBOL ESTERS
TOXICITY
DOSE-RESPONSE RELATIONSHIPS
EMBRYOS
IN VIVO
ANIMALS
BIRDS
CARCINOGENS
ESTERS
FOWL
GROWTH
HORMONES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology