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Title: Renal catabolism of /sup 125/I-glicentin

Abstract

The renal catabolism of /sup 125/I-glicentin has been studied in vivo by the disappearance of this peptide from the plasma of bilaterally nephrectomized, ureteral-ligated, or normal rats and by using tubular microinfusion techniques. In addition the catabolism of glicentin by the isolated, perfused kidney has been studied. Results from in vivo studies demonstrated that half-disappearance time was lower in control (59.5 +/- 1.8 min) than in bilaterally nephrectomized rats (97.2 +/- 2.6 min), and this value was significantly higher than that of ureteral-ligated animals (83.2 +/- 1.1 min, P less than 0.005). Microinfusion experiments revealed that when /sup 125/I-glicentin was injected into the proximal tubule, no trichloroacetic-precipitable radioactivity was recovered in the urine, whereas most of inulin injected was recovered. By contrast most of the /sup 125/I-glicentin injected into the distal tubule was recovered in the urine. In isolated kidney experiments, organ clearance rate of /sup 125/I-glicentin averaged 0.88 +/- 0.10 ml/min, a value significantly higher than that of glomerular filtration rate (0.72 +/- 0.06 ml/min, P less than 0.005, paired data), and both parameters showed a close linear relationship (r = 0.90). Urinary clearance of glicentin was negligible. These results demonstrate that the kidney plays a major role inmore » the catabolism of glicentin, mainly by glomerular filtration and tubular catabolism. The site of tubular catabolism appears to be the proximal tubule. Peritubular uptake was minimal.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Fundacion Jimenez Diaz, Madrid, Spain
OSTI Identifier:
5429162
Resource Type:
Journal Article
Journal Name:
Am. J. Physiol.; (United States)
Additional Journal Information:
Journal Name: Am. J. Physiol.; (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDES; CATABOLISM; RENAL CLEARANCE; BIOCHEMICAL REACTION KINETICS; GLUCAGON; IN VITRO; IODINE 125; KIDNEYS; NEPHRECTOMY; PERFUSED ORGANS; PRECIPITATION; RATS; TRACER TECHNIQUES; URINE; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY; BODY FLUIDS; CLEARANCE; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EXCRETION; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MATERIALS; MEDICINE; METABOLISM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; POLYPEPTIDES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SEPARATION PROCESSES; SURGERY; VERTEBRATES; WASTES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Lopez-Novoa, J M, Santos, J C, Villamediana, L M, Garrote, F J, Thim, L, Moody, A J, and Valverde, I. Renal catabolism of /sup 125/I-glicentin. United States: N. p., 1986. Web.
Lopez-Novoa, J M, Santos, J C, Villamediana, L M, Garrote, F J, Thim, L, Moody, A J, & Valverde, I. Renal catabolism of /sup 125/I-glicentin. United States.
Lopez-Novoa, J M, Santos, J C, Villamediana, L M, Garrote, F J, Thim, L, Moody, A J, and Valverde, I. 1986. "Renal catabolism of /sup 125/I-glicentin". United States.
@article{osti_5429162,
title = {Renal catabolism of /sup 125/I-glicentin},
author = {Lopez-Novoa, J M and Santos, J C and Villamediana, L M and Garrote, F J and Thim, L and Moody, A J and Valverde, I},
abstractNote = {The renal catabolism of /sup 125/I-glicentin has been studied in vivo by the disappearance of this peptide from the plasma of bilaterally nephrectomized, ureteral-ligated, or normal rats and by using tubular microinfusion techniques. In addition the catabolism of glicentin by the isolated, perfused kidney has been studied. Results from in vivo studies demonstrated that half-disappearance time was lower in control (59.5 +/- 1.8 min) than in bilaterally nephrectomized rats (97.2 +/- 2.6 min), and this value was significantly higher than that of ureteral-ligated animals (83.2 +/- 1.1 min, P less than 0.005). Microinfusion experiments revealed that when /sup 125/I-glicentin was injected into the proximal tubule, no trichloroacetic-precipitable radioactivity was recovered in the urine, whereas most of inulin injected was recovered. By contrast most of the /sup 125/I-glicentin injected into the distal tubule was recovered in the urine. In isolated kidney experiments, organ clearance rate of /sup 125/I-glicentin averaged 0.88 +/- 0.10 ml/min, a value significantly higher than that of glomerular filtration rate (0.72 +/- 0.06 ml/min, P less than 0.005, paired data), and both parameters showed a close linear relationship (r = 0.90). Urinary clearance of glicentin was negligible. These results demonstrate that the kidney plays a major role in the catabolism of glicentin, mainly by glomerular filtration and tubular catabolism. The site of tubular catabolism appears to be the proximal tubule. Peritubular uptake was minimal.},
doi = {},
url = {https://www.osti.gov/biblio/5429162}, journal = {Am. J. Physiol.; (United States)},
number = ,
volume = ,
place = {United States},
year = {Thu May 01 00:00:00 EDT 1986},
month = {Thu May 01 00:00:00 EDT 1986}
}