Enzymatic method for radiolabelling vertebrate vitellogenin

Journal Article · Wed Aug 01 00:00:00 EDT 1984 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(84)90180-5· OSTI ID:5424230

Opresko, L; Wiley, H S

Phosphoprotein kinases from Xenopus and chicken liver have been purified and these enzymes have been used to label Xenopus vitellogenin, a phosphoprotein, to high specific activity with (..gamma..-/sup 32/P)ATP. The enzymes were isolated by (NH/sub 4/)/sub 2/SO/sub 4/ fractionation followed by chromatography on DE-52 cellulose and phosphocellulose. This procedure resulted in greater than 20,000-fold enrichment for the enzymes. Both enzyme preparations were used to selectively label vitellogenin in the serum of estrogen-treated animals. Thus, isolation of the vitellogenin prior to radiolabeling was not necessary. The (/sup 32/P)vitellogenin labeled in situ was incorporated by oocytes at a rate similar to (/sup 32/P)vitellogenin labeled in vivo, was translocated to the yolk platelets, and was correctly processed into the yolk proteins.

• Research Organization: Univ. of Utah School of Medicine, Salt Lake City
• OSTI ID: 5424230
• Journal Information: Anal. Biochem.; (United States), Vol. 140:2
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
LABELLED COMPOUNDS
CHEMICAL PREPARATION
PHOSPHOTRANSFERASES
BIOCHEMISTRY
AMPHIBIANS
ATP
CHICKENS
EGGS
ENZYME ACTIVITY
LIVER
PHOSPHORUS 32
PROTEINS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
BODY
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENZYMES
FOWL
GLANDS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
SYNTHESIS
TRANSFERASES
VERTEBRATES
400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
550201 - Biochemistry- Tracer Techniques