Release of leukotrienes C4 and B4 (LTC4, LTB4) and prostaglandin E2 (PGE2) from human monocytes (M phi) induced with aggregated immunoglobulins (Ig) of different classes
Purified human peripheral blood monocytes were stimulated with aggregated human myeloma proteins or the calcium ionophore A23187. Release of LTC4, LTB4 and PGE2 into the supernate was determined by radioimmunoassay and high performance liquid chromatography. The ionophore induced release of 10 +/- 5 ng LTC4 and 25 +/- 8 ng LTB4/10/sup 6/ M phi. Aggregated IgG, IgA and IgE but not IgM or monomeric Ig induced release of LTC4 and LTB4 that was approximately 10-20% of that induced by ionophore. Similarly, IgG, IgA and IgE but not IgM induced release of PGE2 (range 0.015-0.22 ng/10/sup 6/ M phi). Absence of calcium or preincubation with nordihydroguaiaretic acid (10/sup -6/ M) inhibited Ig-induced LTC4 and LTB4 release and indomethacin (10/sup -6/ M) inhibited PGE2 release. Phagocytosis of the Ig aggregates was not required since release was not inhibited by cytochalasin B. Release of PGE2 and LTC4/LTB4 induced by all classes except IgM correlated with the presence or absence of M phi Fc receptors (FcR) for each class as determined by rosette assay. The data indicate that IgG, IgA and IgE immune complexes can induce M phi arachidonic acid metabolism via interaction with FcR despite inhibition of phagocytosis. Such a mechanism may contribute to inflammatory reactions characterized by mononuclear cell infiltrates.
- Research Organization:
- Scripps Clinic and Research Foundation, La Jolla, CA
- OSTI ID:
- 5417663
- Report Number(s):
- CONF-8604222-; TRN: 86-030446
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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ARACHIDONIC ACID
METABOLISM
SECRETION
IMMUNOGLOBULINS
BIOLOGICAL EFFECTS
PROSTAGLANDINS
LIQUID COLUMN CHROMATOGRAPHY
MONOCYTES
PHAGOCYTOSIS
RADIOIMMUNOASSAY
RECEPTORS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
GLOBULINS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LEUKOCYTES
MATERIALS
MEMBRANE PROTEINS
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ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques