Transepithelial transport of glutathione in isolated perfused small intestine
Uptake of GSH was studied in isolated perfused segment of jejunum in the adult rat. Krebs-Henseleit buffer was infused through the superior mesenteric artery and fractions were collected from the portal vein. The maintenance of vascular and epithelial integrity was established by lack of transfer of /sup 14/C-inulin or /sup 14/C-polyethylene glycol from the lumen to the perfusate. (glycine-2-/sup 3/H)GSH was introduced in the lumen and perfusate fractions collected every min. With 1 mM GSH and 10 mM Gly in the lumen, transport into the perfusate was 220 nmol/min. Analysis by HPLC showed that 80% was at the intact tripeptide, GSH. No cysteinylgylcine was detected in the perfusate. Pretreatment of the segment with 0.25 mM acivicin and 1 mM buthionine sulfoximine had no significant effect on GSH transport rate, thus showing that degradation and resynthesis of GSH did not contribute to the appearance of GSH in the perfusate. GSH transport was inhibited 50% by replacing lumenal NaCl with choline Cl. Addition of 10 mM ..gamma..-Clu-Glu or 10 mM ophthalmic acid decreased the rat of transport by 60-70%. These results establish that transepithelial transport of intact GSH occurs in rat small intestine. This may allow utilization of dietary GSH or reutilization of biliary GSH. In addition, the results suggest that oral GSH may be of therapeutic benefit.
- Research Organization:
- Emory Univ. School of Medicine, Atlanta, GA
- OSTI ID:
- 5417223
- Report Number(s):
- CONF-8604222-; TRN: 86-030471
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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GLUTATHIONE
MEMBRANE TRANSPORT
CARBON 14 COMPOUNDS
EPITHELIUM
INULIN
LIQUID COLUMN CHROMATOGRAPHY
PERFUSED TISSUES
POLYETHYLENE GLYCOLS
RATS
SMALL INTESTINE
TRACER TECHNIQUES
URIC ACID
ALCOHOLS
ANIMAL TISSUES
ANIMALS
AROMATICS
AZAARENES
BODY
CARBOHYDRATES
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
GLYCOLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC POLYMERS
ORGANS
PEPTIDES
POLYMERS
POLYPEPTIDES
POLYSACCHARIDES
PROTEINS
PURINES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SACCHARIDES
SEPARATION PROCESSES
TISSUES
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550501* - Metabolism- Tracer Techniques