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Title: /sup 13/C NMR spectroscopy in the analysis of conjugate metabolites in the bile of fish exposed to petroleum

The first natural abundance /sup 13/C NMR investigation of a complex mixture of conjugate metabolites obtained from the gall bladder bile of fish exposed to hydrocarbons is presented. Cunners were exposed to water accommodated No. 2 fuel oil containing about 68% saturates and 22% aromatics. Spectral analysis indicated that the hydrocarbon derivatives were present predominantly as ..beta..-glucuronides, with the oxygen at carbon-1 of glucuronic acid preferentially attached to an aliphatic carbon. The conjugate metabolites were enriched in aromatic-type carbons when compared to the fuel oil or the aromatic fraction of oil.
Authors:
; ;
Publication Date:
OSTI Identifier:
5414709
Resource Type:
Journal Article
Resource Relation:
Journal Name: Chemosphere; (United States); Journal Volume: 15:6
Research Org:
Dept. of Fisheries and Oceans, St. John's, Newfoundland
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BILE; NUCLEAR MAGNETIC RESONANCE; PETROLEUM; BIOLOGICAL EFFECTS; NMR SPECTRA; BILIARY TRACT; CARBON 13; FISHES; METABOLITES; ANIMALS; AQUATIC ORGANISMS; BIOLOGICAL MATERIALS; BODY FLUIDS; CARBON ISOTOPES; DIGESTIVE SYSTEM; ENERGY SOURCES; EVEN-ODD NUCLEI; FOSSIL FUELS; FUELS; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MATERIALS; NUCLEI; RESONANCE; SPECTRA; STABLE ISOTOPES; VERTEBRATES 560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)