Isolation and characterization of a subsurface bacterium capable of growth on toluene, naphthalene, and other aromatic compounds

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5412669

Fredrickson, J K; Brockman, F J; Workman, D J; Li, S W; Stevens, T O ^[1]

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1. Pacific Northwest Lab., Richland, WA (USA)

A bacterium, designated F199, utilized toluene, naphthalene, dibenzothiophene, salicylate, benzoate, p-cresol, and all isomers of xylene as a sole carbon and energy source. This bacterium was isolated from Middendorf sediments, a Cretaceous age formation that underlies the Southeast Coastal Plain in South Carolina, at a depth of approximately 410 m. F199 is a gram-positive, irregular-shaped bacterium that has a varied cell morphology that is dependent on culture medium type and growth stage. F199 required microaerobic conditions (40 to 80 {mu}M O{sub 2}) for growth on hydrocarbons, glucose, acetate, and lactate in mineral salts medium but not for growth on rich media. ({sup 14}C)naphthalene mineralization by F199 was induced by either naphthalene or toluene; however, ({sup 14}C)toluene mineralization by this strain was induced by toluene but not naphthalene. F199 was also found to harbor two plasmids larger than 100 kb. Restricted F199 plasmid and geonomic DNA did not hybridize with toluene (pWWO) or naphthalene (NAH7) catabolic plasmid DNA probes. The presence in the Middendorf formation of bacteria with the capacity for degrading a variety of aromatic compounds suggests that indigenous microorganisms may have potential for in situ degradation of organic contaminants.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5412669
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 57:3; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
POLYCYCLIC AROMATIC HYDROCARBONS
BIODEGRADATION
BENZOIC ACID
BIOASSAY
CARBON 14 COMPOUNDS
CRESOLS
CRETACEOUS PERIOD
DNA
GEOLOGIC FORMATIONS
NAPHTHALENE
PLASMIDS
SALICYLIC ACID
SEDIMENTS
SOUTH CAROLINA
THIONAPHTHENES
TOLUENE
TRACER TECHNIQUES
XYLENES
ALKYLATED AROMATICS
AROMATICS
CARBON COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
DEVELOPED COUNTRIES
FEDERAL REGION IV
GEOLOGIC AGES
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MESOZOIC ERA
MONOCARBOXYLIC ACIDS
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOLS
USA
540211* - Environment
Terrestrial- Basic Studies- Radiometric Techniques- (1990-)