Measurement of iron absorption from meals contaminated with iron
A method is described to measure in vitro the extent of isotopic exchange between the native nonheme food iron and added inorganic reduction to radioiron tracer. The food is digested with pepsin and trypsin in the presence of radioiron. The exchangeability of food iron is calculated from the specific activity in the food and in an extract of bathophenantroline in isoamyl alcohol obtained after digesting this food. The precision and accuracy of the method is illustrated by two kinds of studies, those in which different amounts of contamination iron are added to a meal and those evaluating contamination iron in natural meals. The present method will make it possible to measure validly iron absorption from meals contaminated with unknown amounts of iron of unknown exchangeability with the extrinsic radioiron tracer.
- Research Organization:
- Department of Medicine, University of Goeteborg, Sweden
- OSTI ID:
- 5411813
- Journal Information:
- Am. J. Clin. Nutr.; (United States), Vol. 34:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
FOOD
LABELLING
IRON ISOTOPES
ISOTOPIC EXCHANGE
INTESTINAL ABSORPTION
IRON
PEPSIN
TRACER TECHNIQUES
TRYPSIN
ABSORPTION
ACID PROTEINASES
ELEMENTS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
METALS
PEPTIDE HYDROLASES
SERINE PROTEINASES
TRANSITION ELEMENTS
UPTAKE
400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
400203 - Isotope Exchange & Isotope Separation- (-1987)
550501 - Metabolism- Tracer Techniques