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Title: 5. cap alpha. -reductase activity in rat adipose tissue

We measured the 5 ..cap alpha..-reductase activity in isolated cell preparations of rat adipose tissue using the formation of (/sup 3/H) dihydrotestosterone from (/sup 3/H) testosterone as an endpoint. Stromal cells were prepared from the epididymal fat pad, perinephric fat, and subcutaneous fat of male rats and from perinephric fat of female rats. Adipocytes were prepared from the epididymal fat pad and perinephric fat of male rats. Stromal cells from the epididymal fat pad and perinephric fat contained greater 5..cap alpha..-reductase activity than did the adipocytes from these depots. Stromal cells from the epididymal fat pad contained greater activity than those from perinephric and subcutaneous depots. Perinephric stromal cells from female rats were slightly more active than those from male rats. Estradiol (10/sup -8/ M), when added to the medium, caused a 90% decrease in 5..cap alpha..-reductase activity. Aromatase activity was minimal, several orders of magnitude less than 5..cap alpha..-reductase activity in each tissue studied.
Authors:
; ;
Publication Date:
OSTI Identifier:
5410003
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Soc. Exp. Biol. Med.; (United States); Journal Volume: 186:2
Research Org:
Univ. of Massachusetts Medical School, Worcester (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ADIPOSE TISSUE; ENZYME ACTIVITY; OXIDOREDUCTASES; TESTOSTERONE; BIOSYNTHESIS; ESTRADIOL; FAT CELLS; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANDROGENS; ANDROSTANES; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BODY; CONNECTIVE TISSUE; CONNECTIVE TISSUE CELLS; ENZYMES; ESTRANES; ESTROGENS; HORMONES; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KETONES; LABELLED COMPOUNDS; MAMMALS; ORGANIC COMPOUNDS; RODENTS; SOMATIC CELLS; STEROID HORMONES; STEROIDS; SYNTHESIS; TISSUES; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques