Deleterious effects of endotoxin on cultured endothelial cells: an in vitro model of vascular injury

Journal Article · Mon Jun 01 00:00:00 EDT 1981 · Inflammation (N.Y.); (United States)

DOI:https://doi.org/10.1007/BF00914201· OSTI ID:5407415

Yamada, O; Moldow, C F; Sacks, T; Craddock, P R; Boogaerts, M A; Jacob, H S

The effects of endotoxin-triggered granulocytes on the viability of endothelial cells in vitro was investigated. Endotoxin or its lipid A component caused granulocytes to adhere to and significantly damage cultured endothelial cells. Fresh serum is not necessary but does amplify both adherence and endothelial injury. Much of the endothelial injury was inhibited by free-radical scavengers or by blocking granulocyte adhesion to endothelial cells and appears to result from free radical production by the stimulated granulocyte. Studies in this model suggest a pathogenic role for the endotoxin-triggered granulocyte in the Shwartzman reaction and perhaps related clinical disorders.

• OSTI ID: 5407415
• Journal Information: Inflammation (N.Y.); (United States), Vol. 5:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDOTHELIUM
SENSITIVITY
ENDOTOXINS
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
CHROMIUM ISOTOPES
IN VITRO
LEUKOCYTES
PATHOGENESIS
VIABILITY
ANIMAL TISSUES
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
ISOTOPES
MATERIALS
TISSUES
TOXIC MATERIALS
TOXINS
550200* - Biochemistry
551001 - Physiological Systems- Tracer Techniques
550901 - Pathology- Tracer Techniques