Effect of E coli endotoxin on the leakage of /sup 14/C-sucrose from phosphatidylcholine liposomes

Journal Article · Thu Jan 01 00:00:00 EST 1981 · Circ. Shock; (United States)

OSTI ID:5406780

Onji, T; Liu, M S

The effect of E coli endotoxin on the leakage of /sup 14/C-sucrose from phosphatidylcholine liposomes in the absence or presence of Ca/sup 2 +/ was studied. Endotoxin decreased the leakage from liposomes from 27% to 4% in 5 hr when Ca/sup 2 +/ (1 mM) was incorporated into liposomes during sonication. The effect of endotoxin on the leakiness of liposomes was concentration dependent. Ca/sup 2 +/ alone increased the leakage of /sup 14/C-sucrose from liposomes. Mg/sup 2 +/ at concentrations higher than 5 mM exhibited an effect similar to that of Ca/sup 2 +/. These findings suggest that endotoxin increases the molecular packing of phosphatidylcholine bilayers in the presence of Ca/sup 2 +/ or Mg/sup 2 +/. A change in the physical state of membrane lipid bilayers induced by endotoxin may affect the function of biological membranes.

• OSTI ID: 5406780
• Journal Information: Circ. Shock; (United States), Vol. 8:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON 14 COMPOUNDS
ISOTOPE APPLICATIONS
ENDOTOXINS
BIOLOGICAL EFFECTS
LIPOSOMES
SENSITIVITY
CALCIUM
DOSE-RESPONSE RELATIONSHIPS
ESCHERICHIA COLI
MAGNESIUM
MEMBRANES
PHOSPHOLIPIDS
SACCHAROSE
ALKALINE EARTH METALS
ANTIGENS
BACTERIA
CARBOHYDRATES
CELL CONSTITUENTS
DISACCHARIDES
ELEMENTS
ESTERS
LABELLED COMPOUNDS
LIPIDS
MATERIALS
METALS
MICROORGANISMS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
SACCHARIDES
TOXIC MATERIALS
TOXINS
550201* - Biochemistry- Tracer Techniques
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)