Sequence, tissue distribution, and chromosomal localization of mRNA encoding a human glucose transporter-like protein

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.15.5434· OSTI ID:5405875

Fukumoto, Hirofumi; Seino, Susumu; Imura, Hiroo; Seino, Yutaka; Eddy, R L; Fukushima, Yoshimitsu; Byers, M G; Shows, T B; Bell, G I ^[1]

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1. Univ. of Chicago, IL (USA)

Recombinant DNA clones encoding a glucose transporter-like protein have been isolated from adult human liver and kidney cDNA libraries by cross-hybridization with the human HepG2/erythrocyte glucose transporter cDNA. Analysis of the sequence of this 524-amino acid glucose transporter-like protein indicates that is has 55.5% identity with the HepG2/erythrocyte glucose transporter as well as a similar structural organization. Studies of the tissue distribution of the mRNA coding for this glucose transporter-like protein in adult human tissues indicate that the highest amounts are present in liver with lower amounts in kidney and small intestine. The amounts of glucose transporter-like mRNA in other tissues, including colon, stomach, cerebrum, skeletal muscle, and adipose tissue, were below the level of sensitivity of our assay. The single-copy gene encoding this glucose transporter-like protein has been localized to the q26.1{yields}q26.3 region of chromosome 3.

• OSTI ID: 5405875
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:15; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
GENETIC MAPPING
MESSENGER-RNA
TISSUE DISTRIBUTION
RECOMBINANT DNA
DNA-CLONING
ADIPOSE TISSUE
CEREBRUM
DNA SEQUENCING
ELECTROPHORESIS
GLUCOSE
HETEROCHROMOSOMES
KIDNEYS
LIVER
MAN
MOLECULAR STRUCTURE
MUSCLES
PHOSPHORUS 32
PROTEINS
STOMACH
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHROMOSOMES
CLONING
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DNA
DNA HYBRIDIZATION
GASTROINTESTINAL TRACT
GLANDS
HEXOSES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAPPING
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RNA
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques