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Title: Use of human epidermal cells in the study of carcinogenesis

Because of the importance of human cells, particularly human epithelial cells, in cancer research, we have studied certain phases or events of carcinogenesis using human epidermal cells in primary culture. (1) We found that human epidermal cells are capable of metabolizing benzo(a)pyrene. Large inter-individual variations are found in the basal and induced arylhydrocarbon-hydroxylase activities. (2) UV-induced unscheduled DNA synthesis was demonstrated in human epidermal cells on autoradiographs. We also found that DNA repair is defective in epidermal cells isolated from xeroderma pigmentosum by a new explant-outgrowth culture. (3) Human epidermal cells are unique in that there is a large number of binding sites to phorbol esters compared with mouse epidermal cells, but there is no down-regulation. Further, human epidermal cells show essentially negative responses to tumor promoters, i.e., no stimulation of DNA synthesis, sugar uptake, and no induction of ornithine decarboxylase activity. (4) Human epidermal cells contain 1.5 x 10(5) binding sites per cell for epidermal growth factor (EGF), whereas squamous cell carcinomas of skin and oral cavity have larger amounts of EGF receptors in the order of 10(6) per cell. (5) Based on the above results, we attempted to transform human epidermal cells by the treatment with chemical carcinogens,more » but until now no transformation was obtained. 16 references.« less
Authors:
; ; ;  [1]
  1. (Institute of Medical Science, Tokyo (Japan))
Publication Date:
OSTI Identifier:
5403940
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Investigative Dermatology; (USA); Journal Volume: 92:5 Suppl
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BENZOPYRENE; METABOLISM; GROWTH FACTORS; RECEPTORS; PHORBOL ESTERS; XP CELLS; DNA REPAIR; AUTORADIOGRAPHY; CARCINOGENESIS; CARCINOGENS; CELL CULTURES; CELL DIFFERENTIATION; DNA; EPIDERMIS; EPITHELIUM; MAN; NEOPLASMS; REVIEWS; ULTRAVIOLET RADIATION; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; AROMATICS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BODY; CONDENSED AROMATICS; DISEASES; DOCUMENT TYPES; ELECTROMAGNETIC RADIATION; ESTERS; HYDROCARBONS; MAMMALS; MEMBRANE PROTEINS; MITOGENS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PATHOGENESIS; PRIMATES; PROTEINS; RADIATIONS; RECOVERY; REPAIR; SKIN; TISSUES; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology; 560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture