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Title: Attachment of killed Mycoplasma gallisepticum cells and membranes to erythrocytes

Abstract

To correlate viability with attachment capacity, Mycoplasma gallisepticum cells harvested at different growth phases and treated by various agents were tested for their capacity to attach to human erythrocytes. The results show that viability per se is not essential for M. gallisepticum attachment to erythrocytes, as cells killed by ultraviolet irradiation and membranes isolated by lysing M. gallisepticum cells by various means retained attachment capacity. However, treatment of the mycoplasmas by protein-denaturing agents, such as heart, glutaraldehyde, or prolonged exposure to low pH, drastically affected or even abolished attachment, supporting the protein nature of the mycoplasma membrane components responsible for specific binding to the sialoglycoprotein receptors on the erythrocytes.

Authors:
; ; ;
Publication Date:
Research Org.:
Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
OSTI Identifier:
5403778
Resource Type:
Journal Article
Journal Name:
Infect. Immun.; (United States)
Additional Journal Information:
Journal Volume: 34:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ERYTHROCYTES; RECEPTORS; MYCOPLASMA; CELL MEMBRANES; GLUCOPROTEINS; MAN; PH VALUE; PROTEINS; SIALIC ACID; ULTRAVIOLET RADIATION; VIABILITY; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CELL CONSTITUENTS; ELECTROMAGNETIC RADIATION; MAMMALS; MATERIALS; MEMBRANES; MICROORGANISMS; ORGANIC COMPOUNDS; POLYSACCHARIDES; PRIMATES; RADIATIONS; SACCHARIDES; VERTEBRATES; 550700* - Microbiology; 560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)

Citation Formats

Banai, M, Kahane, I, Feldner, J, and Razin, S. Attachment of killed Mycoplasma gallisepticum cells and membranes to erythrocytes. United States: N. p., 1981. Web.
Banai, M, Kahane, I, Feldner, J, & Razin, S. Attachment of killed Mycoplasma gallisepticum cells and membranes to erythrocytes. United States.
Banai, M, Kahane, I, Feldner, J, and Razin, S. 1981. "Attachment of killed Mycoplasma gallisepticum cells and membranes to erythrocytes". United States.
@article{osti_5403778,
title = {Attachment of killed Mycoplasma gallisepticum cells and membranes to erythrocytes},
author = {Banai, M and Kahane, I and Feldner, J and Razin, S},
abstractNote = {To correlate viability with attachment capacity, Mycoplasma gallisepticum cells harvested at different growth phases and treated by various agents were tested for their capacity to attach to human erythrocytes. The results show that viability per se is not essential for M. gallisepticum attachment to erythrocytes, as cells killed by ultraviolet irradiation and membranes isolated by lysing M. gallisepticum cells by various means retained attachment capacity. However, treatment of the mycoplasmas by protein-denaturing agents, such as heart, glutaraldehyde, or prolonged exposure to low pH, drastically affected or even abolished attachment, supporting the protein nature of the mycoplasma membrane components responsible for specific binding to the sialoglycoprotein receptors on the erythrocytes.},
doi = {},
url = {https://www.osti.gov/biblio/5403778}, journal = {Infect. Immun.; (United States)},
number = ,
volume = 34:2,
place = {United States},
year = {Sun Nov 01 00:00:00 EST 1981},
month = {Sun Nov 01 00:00:00 EST 1981}
}