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Title: Detecting the effects of toxic agents on spermatogenesis using DNA probes

Advances in the molecular biology of spermatogenesis suggest that DNA probes can be used to monitor the effects of toxic agents in male germ cells of mammals. Molecular hybridization analyses with DNA probes can provide a reproducible methodology capable of detecting changes ranging from massive deletions to single base pair substitutions in the genome of exposed individuals. A constantly increasing number of DNA probes that can be used to detect such alterations in human sperm DNA exist for both ubiquitously expressed proteins and for genes solely expressed in the testis. In this chapter, the currently available testicular stage-specific and/or cell type-specific DNA probes and the techniques by which they can be utilized in reproductive toxicology studies are discussed. The advantages, limitations, and future technological advances of this novel biological marker system for the human male reproductive system are also considered.
Authors:
Publication Date:
OSTI Identifier:
5403002
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environ. Health Perspect.; (United States); Journal Volume: 74
Research Org:
Tufts Univ., Medford, MA
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LABELLED COMPOUNDS; HYBRIDIZATION; RECOMBINANT DNA; SPERMATOGENESIS; BIOLOGICAL MARKERS; MOLECULAR BIOLOGY; DNA; ELECTROPHORESIS; GENE MUTATIONS; MALES; MEIOSIS; MESSENGER-RNA; MICE; PHOSPHORUS 32; TOXINS; ANIMALS; ANTIGENS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CELL DIVISION; DAYS LIVING RADIOISOTOPES; GAMETOGENESIS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MATERIALS; MUTATIONS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; RNA; RODENTS; TOXIC MATERIALS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques; 560300 -- Chemicals Metabolism & Toxicology