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Title: Excision repair of UV-induced pyrimidine dimers in human skin in vivo

Abstract

The induction and loss of pyrimidine dimers in human skin in vivo was determined using UV endonuclease, alkaline sucrose sedimentations, and the fluorescent detection of nonradiolabeled DNA. The number of dimers induced following exposure of the skin to radiation emitted from a Burdick UV-800 sunlamp was quantitated by reacting the extracted DNA with Micrococcus luteus endonuclease specific for pyrimidine dimers. Exposure to 15 and 30 seconds of radiation emitted from this lamp produced the formation of 12.8 and 23.6 dimers per 10(8) daltons DNA, respectively. Approximately 50% of the dimers induced were lost 58 min after irradiation. Only a small percentage (less than 10) remained 24 hr postirradiation. These data partially characterize the process by which pyrimidine dimers are excised from human skin DNA in vivo.

Authors:
; ; ;
Publication Date:
OSTI Identifier:
5402367
Resource Type:
Journal Article
Journal Name:
J. Invest. Dermatol.; (United States)
Additional Journal Information:
Journal Volume: 77:3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; DNA; BIOLOGICAL REPAIR; CHEMICAL RADIATION EFFECTS; DIMERS; IN VIVO; NUCLEASES; PYRIMIDINES; SKIN; ULTRAVIOLET RADIATION; AZINES; BIOLOGICAL RECOVERY; BODY; CHEMISTRY; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; HETEROCYCLIC COMPOUNDS; HYDROLASES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHOSPHODIESTERASES; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; 560115* - Radiation Effects on Biochemicals- In Man- (-1987); 560151 - Radiation Effects on Animals- Man; 550200 - Biochemistry

Citation Formats

D'Ambrosio, S M, Slazinski, L, Whetstone, J W, and Lowney, E. Excision repair of UV-induced pyrimidine dimers in human skin in vivo. United States: N. p., 1981. Web. doi:10.1111/1523-1747.ep12482484.
D'Ambrosio, S M, Slazinski, L, Whetstone, J W, & Lowney, E. Excision repair of UV-induced pyrimidine dimers in human skin in vivo. United States. https://doi.org/10.1111/1523-1747.ep12482484
D'Ambrosio, S M, Slazinski, L, Whetstone, J W, and Lowney, E. 1981. "Excision repair of UV-induced pyrimidine dimers in human skin in vivo". United States. https://doi.org/10.1111/1523-1747.ep12482484.
@article{osti_5402367,
title = {Excision repair of UV-induced pyrimidine dimers in human skin in vivo},
author = {D'Ambrosio, S M and Slazinski, L and Whetstone, J W and Lowney, E},
abstractNote = {The induction and loss of pyrimidine dimers in human skin in vivo was determined using UV endonuclease, alkaline sucrose sedimentations, and the fluorescent detection of nonradiolabeled DNA. The number of dimers induced following exposure of the skin to radiation emitted from a Burdick UV-800 sunlamp was quantitated by reacting the extracted DNA with Micrococcus luteus endonuclease specific for pyrimidine dimers. Exposure to 15 and 30 seconds of radiation emitted from this lamp produced the formation of 12.8 and 23.6 dimers per 10(8) daltons DNA, respectively. Approximately 50% of the dimers induced were lost 58 min after irradiation. Only a small percentage (less than 10) remained 24 hr postirradiation. These data partially characterize the process by which pyrimidine dimers are excised from human skin DNA in vivo.},
doi = {10.1111/1523-1747.ep12482484},
url = {https://www.osti.gov/biblio/5402367}, journal = {J. Invest. Dermatol.; (United States)},
number = ,
volume = 77:3,
place = {United States},
year = {Tue Sep 01 00:00:00 EDT 1981},
month = {Tue Sep 01 00:00:00 EDT 1981}
}