Cytotoxicity and mutagenicity of dimethylnitrosamine in mammalian cells (CHO/HGPRT system): enhancement by calcium phosphate
- Oak Ridge National Lab., TN
The cytotoxicity and mutagenicity of dimethylnitrosamine (DMN) was determined in the CHO/HGPRT system. Metabolic activation of the promutagen was achieved by use of a liver homogenate supernatant (S9) prepared from Aroclor 1254-induced Sprague-Dawley rats. The cytotoxic and mutagenic effects of DMN were enhanced by the inclusion of calcium chloride in the incubation mix, and this enhancement was dependent on the presence of sodium phosphate. Under conditions that yielded maximal activity (10 mM calcium chloride, 10 mM magnesium chloride, 50 mM sodium phosphate), an apparent calcium phospate precipitate was observed. DMN activity increased with increasing amounts of S9 protein over the range 0.3-3.0 mg/ml in the S9 mix and appeared to plateau at higher concentrations. The mutagenicity of DMN can be described as 110 mutants/10/sup 6/ cells per mM DMN per mg/ml S9 protein per hour.
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5400953
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 4:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NITROSAMINES
METABOLIC ACTIVATION
MUTAGENESIS
ANIMAL CELLS
CALCIUM PHOSPHATES
HAMSTERS
HOMOGENATES
LIVER
OVARIES
RATS
RESPONSE MODIFYING FACTORS
ALKALINE EARTH METAL COMPOUNDS
AMINES
ANIMALS
BODY
CALCIUM COMPOUNDS
DIGESTIVE SYSTEM
FEMALE GENITALS
GLANDS
GONADS
MAMMALS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
RODENTS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)