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Title: Structure of the major carbohydrate fragment of the Leishmania donovani lipophosphoglycan

Abstract

The major carbohydrate fragment from the lipophosphoglycan of Leishmania donovani was generated by mild acid hydrolysis (0.02 N HCl, 5 min, 100/sup 0/C) and purified by chromatography on DE-52 cellulose and thin layer. By a combination of analyses including gas-liquid chromatography-mass spectrometry and /sup 1/H NMR, the structure of the fragment was elucidated as PO/sub 4/..-->..6Gal(..beta..1-..-->..4)Man. Approximately 16 of these phosphorylated disaccharide units occur in the overall glycoconjugate structure. NMR analysis of an alkaline phosphatase treated phosphorylated tetrasaccharide generated from lipophosphoglycan showed that the phosphorylated disaccharide units are linked together via ..cap alpha..-glycosidic linkages. Complete characterization of the phosphorylated disaccharide units of lipophosphoglycan provides the first example of a defined carbohydrate anchored in membranes by a derivative of phosphatidylinositol.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Kentucky Medical Center, Lexington
OSTI Identifier:
5400329
Resource Type:
Journal Article
Journal Name:
Biochemistry; (United States)
Additional Journal Information:
Journal Volume: 26:19
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; DISACCHARIDES; NUCLEAR MAGNETIC RESONANCE; STRUCTURAL CHEMICAL ANALYSIS; PROTOZOA; BIOCHEMISTRY; ACID HYDROLYSIS; GAS CHROMATOGRAPHY; GLYCOLIPIDS; HEAVY WATER; MASS SPECTROSCOPY; NMR SPECTRA; PARASITES; PHOSPHOLIPIDS; PROTONS; PURIFICATION; THIN-LAYER CHROMATOGRAPHY; TRITIUM COMPOUNDS; ANIMALS; BARYONS; CARBOHYDRATES; CHEMICAL REACTIONS; CHEMISTRY; CHROMATOGRAPHY; DECOMPOSITION; ELEMENTARY PARTICLES; ESTERS; FERMIONS; HADRONS; HYDROGEN COMPOUNDS; HYDROLYSIS; INVERTEBRATES; LABELLED COMPOUNDS; LIPIDS; LYSIS; MAGNETIC RESONANCE; MICROORGANISMS; NUCLEONS; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; OXYGEN COMPOUNDS; RESONANCE; SACCHARIDES; SEPARATION PROCESSES; SOLVOLYSIS; SPECTRA; SPECTROSCOPY; WATER; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550201 - Biochemistry- Tracer Techniques

Citation Formats

Turco, S J, Hull, S R, Orlandi, Jr, P A, Shepherd, S D, Homans, S W, Dwek, R A, and Rademacher, T W. Structure of the major carbohydrate fragment of the Leishmania donovani lipophosphoglycan. United States: N. p., 1987. Web. doi:10.1021/bi00393a042.
Turco, S J, Hull, S R, Orlandi, Jr, P A, Shepherd, S D, Homans, S W, Dwek, R A, & Rademacher, T W. Structure of the major carbohydrate fragment of the Leishmania donovani lipophosphoglycan. United States. https://doi.org/10.1021/bi00393a042
Turco, S J, Hull, S R, Orlandi, Jr, P A, Shepherd, S D, Homans, S W, Dwek, R A, and Rademacher, T W. 1987. "Structure of the major carbohydrate fragment of the Leishmania donovani lipophosphoglycan". United States. https://doi.org/10.1021/bi00393a042.
@article{osti_5400329,
title = {Structure of the major carbohydrate fragment of the Leishmania donovani lipophosphoglycan},
author = {Turco, S J and Hull, S R and Orlandi, Jr, P A and Shepherd, S D and Homans, S W and Dwek, R A and Rademacher, T W},
abstractNote = {The major carbohydrate fragment from the lipophosphoglycan of Leishmania donovani was generated by mild acid hydrolysis (0.02 N HCl, 5 min, 100/sup 0/C) and purified by chromatography on DE-52 cellulose and thin layer. By a combination of analyses including gas-liquid chromatography-mass spectrometry and /sup 1/H NMR, the structure of the fragment was elucidated as PO/sub 4/..-->..6Gal(..beta..1-..-->..4)Man. Approximately 16 of these phosphorylated disaccharide units occur in the overall glycoconjugate structure. NMR analysis of an alkaline phosphatase treated phosphorylated tetrasaccharide generated from lipophosphoglycan showed that the phosphorylated disaccharide units are linked together via ..cap alpha..-glycosidic linkages. Complete characterization of the phosphorylated disaccharide units of lipophosphoglycan provides the first example of a defined carbohydrate anchored in membranes by a derivative of phosphatidylinositol.},
doi = {10.1021/bi00393a042},
url = {https://www.osti.gov/biblio/5400329}, journal = {Biochemistry; (United States)},
number = ,
volume = 26:19,
place = {United States},
year = {Tue Sep 22 00:00:00 EDT 1987},
month = {Tue Sep 22 00:00:00 EDT 1987}
}