Carbon-14 urea breath test for the diagnosis of Campylobacter pylori associated gastritis
Urease in the human gastric mucosa is a marker for infection with Campylobacter pylori (CP), an organism suspected of causing chronic gastritis and peptic ulceration. To detect gastric urease, we examined 32 patients who were being evaluated for possible peptic ulcer disease. Fasting patients were given 10 microCi (370 kBq) of /sup 14/C-labeled urea. Breath samples were collected in hyamine at intervals between 1 and 30 min. The amount of /sup 14/C collected at these times was expressed as: body weight X (% of administered dose of /sup 14/C in sample)/(mmol of CO/sub 2/ collected). The presence of C. pylori colonization was also determined by examination of multiple endoscopic gastric biopsy specimens. On average, patients who were proven to have C. pylori infection exhaled 20 times more labeled CO/sub 2/ than patients who were not infected. The difference between infected patients and C. pylori negative control patients was highly significant at all time points between 2 and 30 min after ingestion of the radionuclide (p less than 0.0001). The noninvasive urea breath is less expensive than endoscopic biopsy of the stomach and more accurate than serology as a means of detecting Campylobacter pylori infection. Because the test detects actual viable CP organisms, it can be used to confirm eradication of the bacterium after antibacterial therapy.
- OSTI ID:
- 5398301
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 29:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIA
GROWTH
DIGESTIVE SYSTEM DISEASES
DIAGNOSIS
UREASE
BIOSYNTHESIS
BACTERIAL DISEASES
CARBON 14 COMPOUNDS
CARBON DIOXIDE
PATIENTS
TRACER TECHNIQUES
UREA
AMIDES
AMIDINASES
CARBON COMPOUNDS
CARBON OXIDES
CARBONIC ACID DERIVATIVES
CHALCOGENIDES
DISEASES
ENZYMES
HYDROLASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
NON-PEPTIDE C-N HYDROLASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SYNTHESIS
550901* - Pathology- Tracer Techniques