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Title: Impaired hepatic handling and processing of lysophosphatidylcholine in rats with liver cirrhosis

Abstract

Lysophosphatidylcholine is a major metabolic product in the plasma and cellular turnover of phospholipids, with well-known membrane-toxic and proinflammatory properties. Because the liver plays a key role in plasma lysophosphatidylcholine removal and biotransformation and because virtually nothing is known of these processes in a diseased organ, the hepatobiliary metabolism of lysophosphatidylcholine was investigated in rats with carbon tetrachloride-induced liver cirrhosis. Twelve adult male Wistar rats with histologically confirmed cirrhosis and 8 control animals were fitted with jugular and biliary catheters and allowed to recover. The animals were kept under constant IV infusion of taurocholate (1 mumol/min). Two microcuries of sn-1{sup 14}Cpalmitoyl-lysophosphatidylcholine was administered as a single bolus. The fate of the injected radioactivity, including removal from plasma, uptake, and subcellular location in the liver and molecular and aggregative forms, was studied by combined chromatographic and radiochemical methods. Major findings were (a) that lysophosphatidylcholine has a prolonged permanence in plasma of cirrhotic rats, due both to decreased hepatic clearance and to depressed conversion into phosphatidylcholine; (b) that the rate of lysophosphatidylcholine acylation is much slower in the cirrhotic than in the normal liver, both at the microsomal and at the cytosolic level; (c) that cytosolic lysophosphatidylcholine in the cirrhotic liver, butmore » not in the normal liver, is predominantly non-protein bound; (d) that the strict molecular selectivity of lysophosphatidylcholine acylation observed in controls is partially lost in cirrhosis; and (e) that a consistent fraction of lysophosphatidylcholine is converted into triacylglycerols in cirrhotics but not in controls.« less

Authors:
; ; ; ; ; ; ; ; ;  [1]
  1. II Division of Gastroenterology, University of Rome La Sapienza (Italy)
Publication Date:
OSTI Identifier:
5394897
Resource Type:
Journal Article
Journal Name:
Gastroenterology; (United States)
Additional Journal Information:
Journal Volume: 101:1; Journal ID: ISSN 0016-5085
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; CARBON TETRACHLORIDE; TOXICITY; LIVER CIRRHOSIS; PATHOGENESIS; PHOSPHOLIPIDS; METABOLISM; ACYLATION; CARBON 14 COMPOUNDS; RATS; TRACER TECHNIQUES; ANIMALS; CARBON COMPOUNDS; CHEMICAL REACTIONS; CHLORINATED ALIPHATIC HYDROCARBONS; DIGESTIVE SYSTEM DISEASES; DISEASES; ESTERS; HALOGENATED ALIPHATIC HYDROCARBONS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPIDS; MAMMALS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; RODENTS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550901 - Pathology- Tracer Techniques

Citation Formats

Angelico, M, Alvaro, D, Cantafora, A, Masella, R, Gaudio, E, Gandin, C, Ginanni Corradini, S, Ariosto, F, Riggio, O, and Capocaccia, L. Impaired hepatic handling and processing of lysophosphatidylcholine in rats with liver cirrhosis. United States: N. p., 1991. Web.
Angelico, M, Alvaro, D, Cantafora, A, Masella, R, Gaudio, E, Gandin, C, Ginanni Corradini, S, Ariosto, F, Riggio, O, & Capocaccia, L. Impaired hepatic handling and processing of lysophosphatidylcholine in rats with liver cirrhosis. United States.
Angelico, M, Alvaro, D, Cantafora, A, Masella, R, Gaudio, E, Gandin, C, Ginanni Corradini, S, Ariosto, F, Riggio, O, and Capocaccia, L. 1991. "Impaired hepatic handling and processing of lysophosphatidylcholine in rats with liver cirrhosis". United States.
@article{osti_5394897,
title = {Impaired hepatic handling and processing of lysophosphatidylcholine in rats with liver cirrhosis},
author = {Angelico, M and Alvaro, D and Cantafora, A and Masella, R and Gaudio, E and Gandin, C and Ginanni Corradini, S and Ariosto, F and Riggio, O and Capocaccia, L},
abstractNote = {Lysophosphatidylcholine is a major metabolic product in the plasma and cellular turnover of phospholipids, with well-known membrane-toxic and proinflammatory properties. Because the liver plays a key role in plasma lysophosphatidylcholine removal and biotransformation and because virtually nothing is known of these processes in a diseased organ, the hepatobiliary metabolism of lysophosphatidylcholine was investigated in rats with carbon tetrachloride-induced liver cirrhosis. Twelve adult male Wistar rats with histologically confirmed cirrhosis and 8 control animals were fitted with jugular and biliary catheters and allowed to recover. The animals were kept under constant IV infusion of taurocholate (1 mumol/min). Two microcuries of sn-1{sup 14}Cpalmitoyl-lysophosphatidylcholine was administered as a single bolus. The fate of the injected radioactivity, including removal from plasma, uptake, and subcellular location in the liver and molecular and aggregative forms, was studied by combined chromatographic and radiochemical methods. Major findings were (a) that lysophosphatidylcholine has a prolonged permanence in plasma of cirrhotic rats, due both to decreased hepatic clearance and to depressed conversion into phosphatidylcholine; (b) that the rate of lysophosphatidylcholine acylation is much slower in the cirrhotic than in the normal liver, both at the microsomal and at the cytosolic level; (c) that cytosolic lysophosphatidylcholine in the cirrhotic liver, but not in the normal liver, is predominantly non-protein bound; (d) that the strict molecular selectivity of lysophosphatidylcholine acylation observed in controls is partially lost in cirrhosis; and (e) that a consistent fraction of lysophosphatidylcholine is converted into triacylglycerols in cirrhotics but not in controls.},
doi = {},
url = {https://www.osti.gov/biblio/5394897}, journal = {Gastroenterology; (United States)},
issn = {0016-5085},
number = ,
volume = 101:1,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1991},
month = {Mon Jul 01 00:00:00 EDT 1991}
}