The importance of residues 195-206 of human blood clotting factor VII in the interaction of factor VII with tissue factor

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.18.7290· OSTI ID:5394331

Wildgoose, P; Kisiel, W; Kazim, A L ^[1]

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1. Univ. of New Mexico, Albuquerque (USA)

Previous studies indicated that human and bovine factor VII exhibit 71% amino acid sequence identity. In the present study, competition binding experiments revealed that the interaction of human factor VII with cell-surface human tissue factor was not inhibited by 100-fold molar excess of bovine factor VII. This finding indicated that bovine and human factor VII are not structurally homologous in the region(s) where human factor VII interacts with human tissue factor. On this premise, the authors synthesized three peptides corresponding to regions of human factor VII that exhibited marked structural dissimilarity to bovine factor VII; these regions of dissimilarity included residues 195-206, 263-274, and 314-326. Peptide 195-206 inhibited the interaction of factor VII with cell-surface tissue factor and the activation of factor X by a complex of factor VIIa and tissue factor half-maximally at concentrations of 1-2 mM. A structurally rearranged form of peptide 195-206 containing an aspartimide residue inhibited these reactions half-maximally at concentrations of 250-300 {mu}M. In contrast, neither peptide 263-274 nor peptide 314-326, at 2 mM concentration, significantly affected either factor VIIa interaction with tissue factor or factor VIIa-mediated activation of factor X. The data provide presumptive evidence that residues 195-206 of human factor VII are involved in the interaction of human factor VII with the extracellular domain of human tissue factor apoprotein.

• OSTI ID: 5394331
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:18; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD COAGULATION
INHIBITION
BLOOD COAGULATION FACTORS
RADIOASSAY
PEPTIDES
MOLECULAR STRUCTURE
BIOSYNTHESIS
CATTLE
DOSE-RESPONSE RELATIONSHIPS
GLYCOPROTEINS
IODINE 125
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
ANIMALS
BETA DECAY RADIOISOTOPES
CHROMATOGRAPHY
COAGULANTS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HEMATOLOGIC AGENTS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RUMINANTS
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques