Quantitative changes in adipocyte plasma membrane in response to nutritional manipulations
The effects of changes in adipocyte size and the effects of nutritional manipulations on the quantity of plasma membrane per adipocyte were investigated. A method for estimating the quantity of plasma membrane was developed based on the specific labeling of adipocyte plasma membrane protein with the nonpermeable labeling agent 125I-labeled diazotized diiodosulfanilic acid. By studying rats (ranging in age from 50 to 125 days) fed a standard laboratory chow or a low fat diet or a high fat diet, a wide range of mean fat cell sizes was obtained. It was found that as the volume of the fat cell increased, the amount of plasma membrane increased in a linear fashion and that this linear relationship had the same slope whether the size of the adipocyte increased slowly with age or rapidly in response to a high fat diet. In contrast, fasting for up to 3 days caused a marked decrease in the mean volume of the adipocytes, but either no change or much less change in the amount of plasma membrane per cell than would have been predicted from the linear relationship between adipocytes, but either no change or much less change in the amount of plasma membrane per cell than would have been predicted form the linear relationship between adipocyte volume and amount of plasma membrane per cell obtained with fed rats, i.e., adipocytes from fasted rats contain more plasma membrane per cell than do fat cells of the same size from fed rats. Neither feeding a high fat diet nor fasting caused detectable changes in the protein and lipid composition of the adipocyte plasma membrane.
- Research Organization:
- Department of Physiology, the University of Texas Health Science Center, San Antonio
- OSTI ID:
- 5394093
- Journal Information:
- J. Lipid Res.; (United States), Vol. 22:7
- Country of Publication:
- United States
- Language:
- English
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CELL MEMBRANES
CYTOLOGY
FAT CELLS
IODINE 125
ISOTOPE APPLICATIONS
ANIMAL FEEDS
DIET
FASTING
LABELLED COMPOUNDS
SULFANILIC ACID
VOLUME
AMINES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOLOGY
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FOOD
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
SOMATIC CELLS
SULFONIC ACIDS
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques