Aneuploidy detection in human sperm nuclei using PRINS technique

Journal Article · Fri Aug 23 00:00:00 EDT 1996 · American Journal of Medical Genetics

DOI:https://doi.org/10.1002/(SICI)1096-8628(19960823)64:3<488::AID-AJMG8>3.3.CO;2-Z· OSTI ID:539406

Girardet, A; Coignet, L; Andreo, B ^[1]

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1. CNRS, Montpellier (France); and others

Rapid and specific identification of chromosomes can be attained in situ using the PRimed IN Situ (PRINS) labelling technique. We have adapted this technique to mature human sperm in combination with a protocol for simultaneous decondensation and denaturation of sperm nuclei. This strategy allowed us to obtain double labelling of human spermatozoa in a <2-hr reaction. In the present study, we report the estimates of disomy for chromosomes 3, 7, 10, 11, and 17 on 64,642 spermatozoa from 2 normal males. The incidences of disomy ranged from 0.28-0.34%. There were no significant interindividual or interchromosomal differences in disomy rates. 27 refs., 1 fig., 3 tabs.

• OSTI ID: 539406
• Journal Information: American Journal of Medical Genetics, Vol. 64, Issue 3; Other Information: PBD: 23 Aug 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
ANEUPLOIDY
DETECTION
SPERMATOZOA
KARYOTYPE
DIPLOIDY
HUMAN CHROMOSOMES
MUTATION FREQUENCY
CHROMOSOMAL ABERRATIONS
NON-DISJUNCTION
MEIOSIS
DNA SEQUENCING
DNA SEQUENCERS
EFFICIENCY
EVALUATION
CELL NUCLEI
IN-SITU HYBRIDIZATION
FLUORESCENCE
PROBES
OLIGONUCLEOTIDES