The peanut lectin-binding glycoproteins of human epidermal keratinocytes

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90459-4· OSTI ID:5393392

Morrison, A I ^[1]; Keeble, S; Watt, F M ^[2]

---

1. Max-Planck-Institute fuer Systemphysiologie, Dortmund (West Germany)
2. Imperial Cancer Research Fund, London (England)

The peanut lectin (PNA) is known to bind more strongly to keratinocytes that are undergoing terminal differentiation than to proliferating keratinocytes. In order to investigate the significance of this change in cell-surface carbohydrate authors have identified the PNA-binding glycoproteins of cultured human keratinocytes and antibodies against them. Two heavily glycosylated bands of 110 and 250 kDa were resolved by PAGE of ({sup 14}C)galactose- or ({sup 14}C)mannose- and ({sup 14}C)glucosamine-labeled cell extracts eluted with galactose from PNA affinity columns. The higher molecular weight band was also detected on PNA blots of unlabeled cell extracts transferred to nitrocellulose. Both bands were sensitive to pronase digestion, but only the 250-kDa band was digested with trypsin. A rabbit antiserum that we prepared (anti-PNA-gp) immunoprecipitated both bands from cell extracts. In contrast to PNA, anti-PNA-gp bound equally to proliferating and terminally differentiating cells, indicating that some epitope(s) of the PNA-binding glycoproteins is present on the cell surface prior to terminal differentiation. When keratinocytes grown as a monolayer in low-calcium medium were switched to medium containing 2 mM calcium ions in order to induce desmosome formation and stratification, there was a dramatic redistribution of the PNA-binding glycoproteins, which became concentrated at the boundaries between cells. This may suggest a role for the glycoproteins in cell-cell interactions during stratification.

• OSTI ID: 5393392
• Journal Information: Experimental Cell Research; (United States), Vol. 177:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
ELECTROPHORESIS
EPIDERMIS
CELL DIFFERENTIATION
GLYCOPROTEINS
BIOCHEMISTRY
CELL CULTURES
CELL PROLIFERATION
GALACTOSE
GLUCOSAMINE
LECTINS
MAN
MANNOSE
SKIN
ALDEHYDES
AMINES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBON COMPOUNDS
CHEMISTRY
EPITHELIUM
HEXOSAMINES
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
SACCHARIDES
TISSUES
VERTEBRATES
550301* - Cytology- Tracer Techniques