Effect of energy intake on the metabolism of glucose and glutamine in rumen epithelial tissue
Ten Holstein steers (579 kg average body weight) were fed either alfalfa hay (12.2% crude protein) or a 90% concentrate diet to supply 14.2 or 25.2 Mcal ME respectively for a minimum of 28 days. Samples of rumen epithelial tissue were removed at slaughter from the anterior ventral sac, washed free of feed particles and transported to the laboratory in oxygenated Krebs-Ringer bicarbonate buffer (KRB; pH 7.4). Papillae were weighed (100-200 mg) in triplicate into flasks containing 3 ml KRB with 1 mM glutamine or 5 mM glucose and acetate (50 mM), propionate (25 mM), butyrate (15 mM), lactate (1 mM) and glucose (5 mM) or glutamine (1 mM) as competing substrates. A parallel set of flasks contained 1 or .5 ..mu..Ci of (U-/sup 14/C)-glutamine or glucose respectively for /sup 14/CO/sub 2/ production. There were no interactions with dietary energy intake and substrate addition. Increasing the dietary energy intake increased (P < .01) rates of uptake, /sup 14/CO/sub 2/ production and net lactate production from glucose and increased the /sup 14/CO/sub 2/ production from glutamine. Addition of acetate, propionate, butyrate and lactate decreased (P < .05) uptake of glucose, but only propionate decreased /sup 14/CO/sub 2/ production from glucose (40%). Addition of butyrate and glucose decreased /sup 14/CO/sub 2/ production from glutamine while propionate addition decreased net glutamate production and increased net alanine production. At these substrate concentrations rates of glucose oxidation to /sup 14/CO/sub 2/ were 7-fold higher than glutamine.
- Research Organization:
- Kansas State Univ., Manhattan
- OSTI ID:
- 5392006
- Report Number(s):
- CONF-8604222-; TRN: 86-028436
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCOSE
METABOLISM
GLUTAMINE
ALANINES
BUTYRIC ACID
CARBON 14 COMPOUNDS
CARBON DIOXIDE
CATTLE
EPITHELIUM
LACTIC ACID
TRACER TECHNIQUES
ALDEHYDES
AMIDES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
DOMESTIC ANIMALS
HEXOSES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RUMINANTS
SACCHARIDES
TISSUES
VERTEBRATES
550501* - Metabolism- Tracer Techniques