Reversibility of disobutamide-induced clear cytoplasmic vacoules in cultured cells following withdrawal of drug
When cultured rat urinary bladder carcinoma cells are incubated with medium containing disobutamide (D), they take up D intracellularly and form clear cytoplasmic vacoules (CCV) which apparently are distended lysosomes containing D. To determine whether CCV are reversible, the authors incubated cells with 10/sup -3/M /sup 14/C-labelled D for 25 hr and cells became full of CCV. Then, cells were incubated in medium free of D and examined at 0,2,4,6,24,48,72 and 144 hr of D withdrawal for radioactivity (600 DPM = 1 ..mu..D), and in situ by phase light microscopy for presence of CCV. The DPM counts at these time points were: 1832, 1539, 1564, 1443, 1073, 738, 481 and 84, respectively. This gradual decrease in cellular content of D parallelled a diminution in size and number of CCV. By 72 hr (481 DPM = 0.93 up D) only few small CCB had remained, and by 144 hr (84 DPM = 0.16 ..mu..g D) CCV had entirely disappeared. Mass spectrometric analysis of the culture medium at 144 hr showed presence of D. These results indicate that (1) the induced CCV are reversible (2) reversibility is associated is associated with release of D from cells (3) the principle compound released from cells is D and not its metabolite(s). The results support the interpretation that CCV are formed as a result of intracellular storage of D.
- Research Organization:
- R and D G.D. Searle and Co., Skokie, IL
- OSTI ID:
- 5388344
- Report Number(s):
- CONF-8604222-; TRN: 86-028560
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Pulmonary alveolar macrophages (PAM) engulf and regain elastin particles and do not respond to some stimuli of neutrophil (PMN) elastinolysis
EFFECTS OF CONTINUOUS LOW LEVEL COBALT 60 GAMMA RADIATION ON A MAMMALIAN CELL SYSTEM
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BLADDER
CARCINOMAS
DIURETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL CULTURES
CYTOPLASM
MASS SPECTROSCOPY
RATS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BODY
DISEASES
DRUGS
MAMMALS
NEOPLASMS
ORGANS
RODENTS
SPECTROSCOPY
URINARY TRACT
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550901 - Pathology- Tracer Techniques