Inhibition of Na/sup +/-dependent uptake of hexose and amino acids in LLC-PK1 cells by amiloride and amiloride-derivatives
Post-confluent LLC-PK1 cells, a renal epithelial cell line from pig kidney, have at their apical borders a Na/sup +/-dependent transport system for hexoses (assayed by the phlorizin-sensitive uptake of alpha-methylglucoside) and at their basolateral surfaces a Na/sup +/-dependent A-system transporter for amino acids (assayed by the uptake of methylaminoisobutyric acid). Amiloride at high concentrations (4.5 mM) partially inhibits both of these transporters. The analogs 5-(N-methyl-N-isobutyl) amiloride and 5-(N-ethyl-N-isopropyl) amiloride can completely inhibit both systems with inhibitory coefficients of approximately 50 ..mu..M in 150 mM NaCl media. Na/sup +//H/sup +/ exchange is also inhibited in these cells by these compounds, but at much lower concentrations. The inhibition of transport of the hexose is competitive with Na/sup +/ in the assay medium at Na/sup +/ concentrations below 10 mM.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 5388270
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINO ACIDS
MEMBRANE TRANSPORT
DIURETICS
BIOLOGICAL EFFECTS
HEXOSES
EPITHELIUM
INHIBITION
ION EXCHANGE
KIDNEYS
SODIUM COMPOUNDS
SWINE
UPTAKE
ALKALI METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
DOMESTIC ANIMALS
DRUGS
MAMMALS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
SACCHARIDES
TISSUES
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)