Human T cell activation. III. Induction of an early activation antigen, EA 1 by TPA, mitogens and antigens

Hara, T; Jung, L K.L.; FU, S M

Title: Human T cell activation. III. Induction of an early activation antigen, EA 1 by TPA, mitogens and antigens

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Abstract

With human T cells activated for 12 hours by 12-o-tetradecanoyl phorbol-13-acetate (TPA) as immunogen, an IgG/sub 2a/ monoclonal antibody, mAb Ea 1, has been generated to a 60KD phosphorylated protein with 32KD and 28KD subunits. The antigen, Ea 1, is readily detected on 60% of isolated thymocytes by indirect immunofluorescence. A low level of Ea 1 expression is detectable on 2-6% of blood lymphocytes. Isolated T cells have been induced to express Ea 1 by TPA, mitogens and anitgens. TPA activated T cells express Ea 1 as early as 1 hour after activation. By 4 hours, greater than 95% of the T cells stain with mAb Ea 1. About 50% of the PHA or Con A activated T cells express Ea 1 with a similar kinetics. Ea 1 expression proceeds that of IL-2 receptor in these activation processes. T cells activated by soluble antigens (tetanus toxoid and PPD) and alloantigens in MLR also express Ea 1 after a long incubation. About 20% of the T cells stain for Ea 1 at day 6. Ea 1 expression is not limited to activated T cells. B cells activated by TPA or anti-IgM Ab plus B cell growth factor express Ea 1. Themore »« less

Authors:: Hara, T; Jung, L K.L.; FU, S M

Publication Date:: Sat Mar 01 00:00:00 EST 1986

Research Org.:: Oklahoma Medical Research Foundation, Oklahoma City

OSTI Identifier:: 5388070

Report Number(s):: CONF-8604222-
Journal ID: CODEN: FEPRA

Resource Type:: Conference

Journal Name:: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

Additional Journal Information:: Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOCYTES; BIOCHEMICAL REACTION KINETICS; PHORBOL ESTERS; BIOLOGICAL EFFECTS; ANTIGENS; FLUORESCENCE; LYMPHOKINES; MITOGENS; MONOCLONAL ANTIBODIES; PROTEINS; RECEPTORS; ANIMAL CELLS; ANTIBODIES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARCINOGENS; CONNECTIVE TISSUE CELLS; ESTERS; GROWTH FACTORS; KINETICS; LEUKOCYTES; LUMINESCENCE; MATERIALS; MEMBRANE PROTEINS; ORGANIC COMPOUNDS; REACTION KINETICS; SOMATIC CELLS; 560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)

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                    Hara, T, Jung, L K.L., and FU, S M. Human T cell activation. III. Induction of an early activation antigen, EA 1 by TPA, mitogens and antigens.  United States: N. p., 1986. 
        Web.

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                    Hara, T, Jung, L K.L., & FU, S M. Human T cell activation. III. Induction of an early activation antigen, EA 1 by TPA, mitogens and antigens.  United States.

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                    Hara, T, Jung, L K.L., and FU, S M. 1986.  
        "Human T cell activation. III. Induction of an early activation antigen, EA 1 by TPA, mitogens and antigens".  United States.

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@article{osti_5388070,

  title        = {Human T cell activation. III. Induction of an early activation antigen, EA 1 by TPA, mitogens and antigens},

  author       = {Hara, T and Jung, L K.L. and FU, S M},

  abstractNote = {With human T cells activated for 12 hours by 12-o-tetradecanoyl phorbol-13-acetate (TPA) as immunogen, an IgG/sub 2a/ monoclonal antibody, mAb Ea 1, has been generated to a 60KD phosphorylated protein with 32KD and 28KD subunits. The antigen, Ea 1, is readily detected on 60% of isolated thymocytes by indirect immunofluorescence. A low level of Ea 1 expression is detectable on 2-6% of blood lymphocytes. Isolated T cells have been induced to express Ea 1 by TPA, mitogens and anitgens. TPA activated T cells express Ea 1 as early as 1 hour after activation. By 4 hours, greater than 95% of the T cells stain with mAb Ea 1. About 50% of the PHA or Con A activated T cells express Ea 1 with a similar kinetics. Ea 1 expression proceeds that of IL-2 receptor in these activation processes. T cells activated by soluble antigens (tetanus toxoid and PPD) and alloantigens in MLR also express Ea 1 after a long incubation. About 20% of the T cells stain for Ea 1 at day 6. Ea 1 expression is not limited to activated T cells. B cells activated by TPA or anti-IgM Ab plus B cell growth factor express Ea 1. The kinetics of Ea 1 expression is slower and the staining is less intense. Repeated attempts to detect Ea 1 on resting and activated monocytes and granulocytes have not been successful. Ea 1 expression is due to de novo synthesis for its induction is blocked by cycloheximide and actinomycin D. Ea 1 is the earliest activation antigen detectable to-date.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5388070},
  journal      = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number       = ,

  volume       = 45:3,

  place        = {United States},

  year         = {Sat Mar 01 00:00:00 EST 1986},

  month        = {Sat Mar 01 00:00:00 EST 1986}

}

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