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Title: Mutagenic azide metabolite is azidoalanine

Sodium axide produces high mutation rates in a number of species. Azide mutagenicity is mediated through a metabolite in barley and bacteria. Many studies showed that azide affects the L-cysteine biosynthesis pathway. Cell-free extracts of Salmonella typhimurium convert azide and O-acetylserine to the mutagenic metabolite. O-acetylserine sulfhydrylase was identified as the enzyme responsible for the metabolite biosynthesis. To confirm the conclusion that the azide metabolite is formed through the ..beta..-substitution pathway of L-cysteine, we radioactively labeled the azide metabolite using /sup 14/C-labeled precursors. Moreover, the mutagenic azide metabolite was purified and identified as azidoalanine based on mass spectroscopy and elemental analysis. 26 refs., 3 figs., 1 tab.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
5385325
Report Number(s):
DOE/EV/72002-59
ON: DE86015353
DOE Contract Number:
AT06-76EV72002
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Research Org:
Washington State Univ., Pullman (USA); Yarmouk Univ., Irbid (Jordan). Dept. of Biological Sciences
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; AZIDES; METABOLITES; MUTAGENESIS; ALANINES; CARBON 14 COMPOUNDS; EXPERIMENTAL DATA; INFRARED SPECTRA; MASS SPECTROSCOPY; SALMONELLA; THIN-LAYER CHROMATOGRAPHY; TRACER TECHNIQUES; AMINO ACIDS; BACTERIA; CARBOXYLIC ACIDS; CHROMATOGRAPHY; DATA; INFORMATION; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROORGANISMS; NITROGEN COMPOUNDS; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC COMPOUNDS; SEPARATION PROCESSES; SPECTRA; SPECTROSCOPY 560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987); 550501 -- Metabolism-- Tracer Techniques