Bile salt-stimulated lipase of human milk: characterization of the enzyme from preterm and term milk
The bile salt-stimulated lipase (BSSL) of human milk is an important digestive enzyme in the newborn whose pancreatic function is immature. Milk from mothers delivering premature infants (preterm milk) has similar levels of BSSL activity to that of mothers of term infants (term milk). This study has determined whether the BSSL in preterm milk has the same characteristics as that in term milk. Milk samples were collected during the first 12 wk of lactation from seven mothers of infants born at 26-30 wk (very preterm, VPT), 31-37 wk (preterm, PT) and 37-42 wk (term, T) gestation. BSSL activity was measured using /sup 3/H-triolein emulsion as substrate. Time course, bile salt and enzyme concentration, pH and pH stability were studied, as well as inhibition of BSSL by eserine. The characteristics of BSSL from preterm and term milk were identical as were comparisons between colostrum and mature milk BSSL. BSSL from all milk sources had a neutral-to-alkaline pH optimum (pH 7.3-8.9), was stable at low pH for 60 min, and was 95-100% inhibited by eserine (greater than or equal to 0.6 mM). BSSL activity, regardless of enzyme source, was bile-salt dependent and was stimulated only by primary bile salts (taurocholate, glycocholate). The data indicate that the BSSL in milks of mothers delivering as early as 26 wk gestation is identical to that in term milk.
- Research Organization:
- Georgetown Univ. Medical Center, Washington, DC
- OSTI ID:
- 5385218
- Report Number(s):
- CONF-8604222-; TRN: 86-026609
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPASE
CHEMICAL COMPOSITION
ENZYME ACTIVITY
BILE
LACTATION
MAN
MILK
PH VALUE
TRACER TECHNIQUES
TRIOLEIN
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOXYLESTERASES
ENZYMES
ESTERASES
ESTERS
FOOD
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MATERIALS
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PRIMATES
TRIGLYCERIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques