Bile salt-stimulated lipase: an animal model for human lactation
To date, bile salt-stimulated lipase (BSSL), an important digestive enzyme for the newborn, has only been described in the milk of primates - human and gorilla. The authors report the presence of BSSL in milks of dog and cat. Serial collections from two dogs (day 1-49) and cats (day 5-57) were analyzed for BSSL activity using a /sup 3/H-triolein emulsion as substrate. Comparable analyses of pooled, term human milk were made for comparison. BSSL activity in individual dog milks (x = 32.0; range: 4.8-107.4 U/ml) was similar, while that in cat milk (x = 6.6; range: 2.2-16.9 U/ml) was lower than in human milk (x = 37.0; range: 10-80 U/ml; n = 35). Longitudinal patterns for BSSL differed depending upon the enzyme source. Dog, cat and human milk BSSL all showed a neutral to alkaline pH optimum (pH 7.0-8.4), stability at low pH, and 95-100% inhibition (at concentrations of 0.6 mM) by NaCl and eserine. BSSL activity from all sources had an obligate requirement for primary bile salts. These data are the first to show BSSL activity in milk from mammals other than human and gorilla. Presence of BSSL in nonprimate milk will permit the careful study of BSSL biology in the mammary gland as well as its role in neonatal fat digestion.
- Research Organization:
- Georgetown Univ. Medical Center, Washington, DC
- OSTI ID:
- 5385181
- Report Number(s):
- CONF-8604222-; TRN: 86-026610
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPASE
ENZYME ACTIVITY
BILE
BIOLOGICAL MODELS
CATS
COMPARATIVE EVALUATIONS
DOGS
LACTATION
MAMMARY GLANDS
MAN
MILK
PH VALUE
TRACER TECHNIQUES
TRIOLEIN
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOXYLESTERASES
ENZYMES
ESTERASES
ESTERS
FOOD
GLANDS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MATERIALS
OILS
ORGANIC COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
PRIMATES
TRIGLYCERIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques