Effect of hemoglobin on the uptake of /sup 3/H-norepinephrine and /sup 3/H-choline chloride into porcine cerebral arteries
Prolonged constriction of cerebral arteries often follows subarachnoid hemorrhage (SAH). SAH exposes hemoglobin (Hb) to cerebral arteries and Hb has been demonstrated to induce vasoconstriction as well as alter cerebrovascular neurogenic response characteristics. The effect of Hb on uptake of /sup 3/H-norepinephrine (/sup 3/H-NE) and /sup 3/H-choline chloride (/sup 3/H-ChCl) into porcine cerebral arteries was therefore examined. 0.5 to 50 ..mu..M porcine Hb caused a dose-dependent inhibition of /sup 3/H-NE uptake into the anterior (ANT), internal carotid (IC) and middle cerebral (MC) arteries of the pig. IC/sub 50/ values for uptake inhibition were: ANT, 31 ..mu..M; IC, 34 ..mu..M; MC, 37 ..mu..M. Porcine serum albumin (PSA) in the same concentration range also caused a decrease in /sup 3/H-NE uptake. An examination of protein-ligand interactions using column chromatography demonstrated binding of /sup 3/H-NE by both Hb and PSA. This protein binding may be responsible for part of the uptake inhibition. Hb and PSA had little effect on /sup 3/H-ChCl uptake into these arteries.
- Research Organization:
- Southern Illinois Univ. School of Medicine, Springfield
- OSTI ID:
- 5383284
- Report Number(s):
- CONF-8604222-; TRN: 86-026734
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Decreased norepinephrine (NE) uptake in cerebral cortex and inferior colliculus of genetically epilepsy prone (GEP) rats
Vascular effects of acetylcholine in the perfused rabbit lung
Related Subjects
ALBUMINS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
ARTERIES
VASOCONSTRICTION
CHOLINE
UPTAKE
HEMOGLOBIN
NORADRENALINE
DOSE-RESPONSE RELATIONSHIPS
HEMORRHAGE
LIQUID COLUMN CHROMATOGRAPHY
SWINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CHROMATOGRAPHY
DOMESTIC ANIMALS
DRUGS
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PIGMENTS
PORPHYRINS
PROTEINS
QUATERNARY COMPOUNDS
REACTION KINETICS
SEPARATION PROCESSES
STEROID HORMONES
SYMPATHOMIMETICS
SYMPTOMS
VERTEBRATES
550901* - Pathology- Tracer Techniques